摘要
目的建立醋酸曲安奈德樟脑搽剂中樟脑含量的测定方法。方法使用外标法,样品处理过程中加入二甲基亚砜,明显改善峰型,采用极性毛细管柱程序升温的方法。色谱柱:Rtx-Wax毛细管(30 m×0.25 mm×0.25μm)柱;进样口温度200℃,氢火焰离子化检测器(FID)温度250℃,初始柱温为50℃,保持3 min,以10℃/min升到110℃,保持1 min。结果待测物樟脑峰面积与质量浓度均呈良好的线性关系,精密度RSD值为0.2%;平均回收率为98.8%～100.8%。结论气相色谱法简便、准确、灵敏度高,优于药典内标法,可用于醋酸曲安奈德樟脑搽剂中樟脑含量的测定。
Objective To establish a method for the determination of camphor in triamcinolone acetonide acetate and camphor liniment.Methods Using the external standard method,adding dimethyl sulfoxide in the process of sample treatment,the peak shape was improved obviously,and the method of polarity capillary column temperature programmed was adopted.Column:Rtx-Wax(30 m×0. 25 mm×0.25 μm),inlet temperature:200 ℃,FID detector temperature:250 ℃,the initial column temperature is 50 ℃,3 min,10 ℃/min to rise to 110 ℃,1 min.Results The camphor peak area and the mass concentration of the tested samples showed a good linear relationship,the precision RSD value was 0.2%,and the average recovery rate was 98.8%~100.8%.Conclusion Gas chromatography is simple,accurate and sensitive,it is superior to the standard internal standard method for the determination of camphor,and it can be used for the determination of camphor in triamcinolone acetonide acetate camphor liniment.
作者
陈思明
林景
李园
林舒平
朱小林
CHEN Siming;LIN Jing;LI Yuan(Hainan Provincial Food and Drug Inspection Institute,Wuzhishan Branch,Hainan Province,Wuzhishan 572299,China)
出处
《临床合理用药杂志》
2019年第4期54-55,57,共3页
Chinese Journal of Clinical Rational Drug Use
基金
海南省自然科学基金(No:20163150)
关键词
醋酸曲安奈德樟脑搽剂
气相色谱法
樟脑含量测定
Triamcinolone acetonide acetate camphor liniment
Gas chromatography
Determination of camphor content
