摘要
用苗龄10d的花生无菌苗的上胚轴为外植体,置于MS+TDZ1mg/L+BA2mg/L+NAA0 5mg/L的培养基中,14d左右,可见丛生芽分化,45d后长出整齐一致的丛生芽,诱导率达80 2%.平均出芽数达9 1个.同时添加YE与AD和适当浓度的AgNO3,外植体的出芽率提高到90%,切取诱导芽单芽,转接至含有NAA的根诱导培养基中,在同样的培养条件下,30d后,G4号培养基中的外植体出根率达80%,且根粗壮,较长.
Adventitious shoots were induced directly from the epicotyl of peanut (Arachis hypogaea) taken from the 10 days old aseptic seedling of peanut. The explants were cultured on media with MS and TDZ 1 mg/L+BA 2 mg/L+NAA 0.5 mg/L . 14 days later, the adventitious shoots began to differentiate incessantly, and to 45 days, all explants differentiated normal regular. The rate of organogenesis was 80.2%. There was 9.1 shoots per explant in average. The shoots were isolated and the single shoot was taken out and cultured on root induction media with NAA in the same conditions mentioned above and 30 days later, the rooting rate of explants on medium G4 was 80%, and the root was stronger and longer.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2003年第3期46-49,共4页
Journal of South China Agricultural University
基金
广东省自然科学基金资助项目(2KM02920N)
