摘要
选择不同基因型的花生品种为外植体供体,以初步建立适应河南花生品种的高效再生体系。以5天苗龄的花生无菌胚轴为外植体,将供试的4个花生品种分别接种于4种丛生芽诱导培养基上:MS+6-BA 5 mg/L+NAA 1 mg/L,MS+TDZ 0.6 mg/L+NAA 0.4 mg/L,MS+TDZ 1 mg/L+6-BA 1 mg/L+NAA0.5 mg/L,MS+TDZ 1 mg/L+6-BA 2 mg/L+NAA 0.5 mg/L。在25℃±1℃、2000 lx、16 h/d光照条件下培养约30天左右,上胚轴和下胚轴均分化出愈伤组织和丛生芽点。结果发现,上胚轴的丛生芽诱导率远高于下胚轴,最高达到67%,平均每个外植体产生4.5个丛生芽,最高的可分化出30多个;上胚轴在培养基MS+6-BA 5 mg/L+NAA 1 mg/L和MS+TDZ 1 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L的丛生芽分化较好,该研究为花生组织的离体培养和外植体遗传转化提供有效途径。
In this study, explants from different genetic peanut（Arachis hypogaea L.） varieties were induced soas to establish a more efficient plant regeneration system of peanut cultivars which were only adaptable inHenan Province. Four peanut cultivars were selected as research materials, embryoids developed from the 5-day-old seedlings of these cultivars on MS basal medium were used as the explants, and then cultured on MSsupplemented with 4 different ratios of hormone： MS＋ 6-BA 5 mg/L＋ NAA 1 mg/L, MS＋TDZ 0.6 mg/L＋NAA0.4 mg/L, MS＋TDZ 1 mg/L＋6-BA 1 mg/L＋NAA 0.5 mg/L, MS＋TDZ 1 mg/L＋6-BA 2 mg/L＋NAA 0.5 mg/L.Being cultured about 30 d under the temperature of 25℃±1℃ with the illumination of 2000 lx lasted for 16 h,the morphogenetic callus and shoots regeneration could be found on both epicotyl and hypocotyl of peanutexplants. The average percentage of epicotyl shoots was up to 67%, and the average number of shoots perexplant was 4.5 per explant, the most one could be up to 30 per explant, which was higher than that ofhypocotyls. Epicotyl had a higher ratio of inducing production on MS＋6-BA 5 mg/L＋ NAA 1 mg/L and TDZ1 mg/L＋6-BA 1 mg/L＋NAA 0.5 mg/L. As a result, an effective way of plant regeneration in vitro and genetictransformation was established.
出处
《中国农学通报》
2015年第21期149-156,共8页
Chinese Agricultural Science Bulletin
基金
现代农业产业技术体系建设"国家花生产业技术体系"(CARS-14)
农业部农业科研杰出人才及其创新团队项目
河南省农业科学院科研发展专项资金项目(201218305)
关键词
胚轴
诱导
丛生芽
花生
embryo axe
induction
adventitious shoots
peanut
