摘要
以花生品种远杂9102成熟种胚发芽12d、4d的幼叶为外植体,对花生幼叶不定芽诱导制约因素进行了研究,结果表明,采用较高浓度的6-BA(8mg/L)、较低浓度的NAA(1mg/L),不定芽诱导率可达70%以上。最佳诱导培养基为MS+6-BA8mg/L+NAA0.5mg/L+AgNO32mg/L;最佳继代培养基为MS+6-BA5mg/L+NAA2mg/L+AgNO32mg/L。同时试验发现种子预培养4d的幼叶不定芽诱导率较预培养12d的高;花生幼叶近叶柄基部切口处不定芽诱导率较高,是较理想的不定芽诱导部位。以远杂9102预培养2d的子叶作外植体,对愈伤组织诱导及分化进行试验,确定MS+6-BA4.5mg/L+2,4-D2.2mg/L为诱导愈伤的最佳培养基,愈伤组织诱导率达79.8%,最佳分化培养基为MS+KT(0.15mg/L)。
The restrictive factors for adventitious bud induction from leaflet were investigated using the 12d and 4d seedlings of peanut variety Yuanza 9102. Results showed that the adventitious bud induction rate reached to 70% in the MS added with high concentrations of 6-BA（8mg/L）, low concentrations of NAA（1mg/L）, indicating that the MS medium＋8mg/L 6-BA＋0.5mg/L NAA＋2mg/L AgNO3 had the best result for inducing the adventitious buds. The best medium for subculture was MS＋5mg/L 6-BA＋2mg/L NAA＋2mg/L AgNO3. And the adventitious bud induction rate of 4d seedlings of peanut was higher than the 12d. It was also observed that the underside cut near the petiole presented a higher rate of adventitious buds, so was the ideal explant for adventitious bud induction. The tissue culture of peanut was studied using 2d cotyledon explant of peanut variety Yuanza 9102. The result showed that the best medium for the induction of callus was MS＋6-BA4.5mg/L＋2,4-D 2.2mg/L, The callus induction rate reached to 79.8%. And MS＋KT （0.15mg/L） was the best differentiation medium.
出处
《花生学报》
2009年第4期9-14,共6页
Journal of Peanut Science
基金
河南省科技攻关项目(072102120021)
关键词
花生
子叶
组织培养
不定芽诱导
植株再生
peanut cotyledon tissue culture adventitious buds induction regeneration
