摘要
目的 : 研究大豆、枸杞子、山楂复合提取物对小鼠化学性肝损伤的保护作用。方法 : 设立空白对照、肝损伤对照和 0 .0 6、0 .2 0、0 .60 g/( kg· bw)复合提取物五组 ,分别建立小鼠CCl4 肝损伤模型和乙醇肝损伤模型 ,4w后 ,前者测定血清中丙氨酸氨基转移酶 ( ALT)及天门冬氨酸氨基转移酶 ( AST)活性 ,同时观察肝脏组织病理 ;后者测定肝匀浆中丙二醛 ( MDA)、谷胱甘肽( GSH)和甘油三酯 ( TG)含量 ,同时观察肝脏病理。结果 : CCl4 肝损伤模型 :复合提取物中剂量组的血清 ALT及中、高剂量组的血清 AST水平均较 CCl4 对照组降低 ,低剂量组发生肝细胞坏死和中、高剂量组发生炎症细胞浸润的程度明显较 CCl4 对照组轻。乙醇肝损伤模型 :低、高剂量组MDA和各剂量组 TG含量以及低、中剂量组脂肪变性评分均明显低于乙醇对照组。结论 : 大豆、枸杞子、山楂复合提取物对 CCl4 和乙醇诱导的小鼠化学性肝损伤有保护作用。
Objective: To study the protective effect of compound extract of soybean, wolfberry and hawthorn on chemical liver injury in mice respectively. Methods:The mice were randomly divided into 5 groups:control(A), liver injury control(B)and 0.06(C)?0.20(D)?0.60(E) g/(kg·bw) compound extract group. The liver injury models were induced by CCl 4 and ethanol in mice respectively. After 4 weeks, for the former, the serum ALT and AST content were measured and the liver pathological change was observed. For the latter, the liver MDA, GSH, and TG content were measured and fat droplets staining in the ice pathological slice were observed . Results: 1.CCl 4 liver injury models:the content of serum ALT of group D and the AST of group D and E were decreased. Compared with normal group, the hepatic pathological changes of the compound extract group was mainly fatty and ballon degeneration, and that of the CCl 4 group was mainly hepatic cytoclasis. 2.Ethanol liver injury models: the content of liver MDA of group C, E and the TG of group C, D, E were all decreased, and the fatty degeneration scores of group C, D were lower, both compared with ethanol group. Conclusion: Compound extract of soybean, wolfberry and hawthorn has the protective effect on liver injuries induced by both CCl 4 and ethanol in mice.
出处
《营养学报》
CAS
CSCD
北大核心
2003年第2期208-211,共4页
Acta Nutrimenta Sinica
