摘要
目的:探讨维生素C(VC)和维生素E(VE)对CCl_4引起化学性肝损伤的预防性保护作用.方法:昆明种小鼠60只随机分5组,设正常对照组、病理模型组、VC保护组、VE保护组、VC+VE保护组,饲养10 d,除正常对照组外,其余各组ip 1.5 mL/L CCl_4致小鼠化学性肝损伤,测定小鼠血清中ALT,AST及肝细胞中MDA,GSH,SOD,HE染色光镜下观察肝细胞形态变化.结果:VC和VE保护组能显著降低血清中ALT和AST(2277.12±1187.90,2163.76±1412.11 nkat/L vs 4527.07±1019.37 nkat/L,P<0.01)以及肝细胞中脂质过氧化物MDA的含量(4.37±0.49,3.26±0.71μmol/g vs 9.25±2.74μmol/g,P<0.01).镜下观查肝损伤明显减轻,体外抗氧化实验能显著性的抑制脂质过化物MDA生成,联合应用有协同效应.结论:VC和VE对化学性肝损伤有预防性保护作用.
AIM： To find out the protective effect of vitamin C （VC） and vitamin E （VE） against chemical liver injury caused by carbon tetrachloride （CCl4）.METHODS： A total of 60 Kunming rats were randomly divided into 5 groups： control group, model group, VC-treated group, VE-treated group, and VC plus VE group. After 10 days of treatment, 1.5 mL/L CCl4 were injected into all the mice except those in control group to induce liver injury. Finally, the serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and tissue contents of malondialdehyde （MDA）, glutathione （GSH） and superoxide dismutase （SOD） were examined. The morphological changes of liver tissues were observed under light microscope.RESULTS： VC and VE significantly decreasedthe levels of serum ALT and AST （2277.12 ± 1187.90, 2163.76 ± 1412.11 nkat/L vs 4527.07 ± 1019.37 nkat/L, P 〈 0.01）, and tissue content of MDA （4.37 ± 0.49, 3.26 ± 0.71 μmol/g vs 9.25 ± 2.74 μmol/g, P 〈 0.01）. Under microscope, liver injury was greatly decreased in VC and VB group, especially in VC＋VB group. CONCLUSION： Both VC and VE can prevent liver tissues from chemical injury, and combined use presents a better effect.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第14期1646-1649,共4页
World Chinese Journal of Digestology
基金
郧阳医学院中青年基金项目
No.2005ZQY02~~
