摘要
通过缓冲液浸提、戊二醛固定化红细胞吸附、亲和层析和凝胶过滤等方法 ,从家蝇蛹中分离纯化出一种对半乳糖专一的凝集素 .结果表明 ,亲和层析后 ,SDS 聚丙烯酰胺凝胶电泳检测家蝇蛹凝集素 (MPL)分子质量为 84ku .SephadexG 2 0 0凝胶过滤后 ,MPL分子质量为 86ku .家蝇蛹凝集素专一性识别D 半乳糖 ,其血凝活力对热不稳定 ,不依赖Ca2 +,在 pH 6～ 9之间稳定 .家蝇蛹凝集素对Escherichiacoli。
A new galactose-specific lectin (MPL) from Musca domestic. pupae was isolated. The purification procedure entailed extraction with aqueous buffer, GRBC (glutaraldehydeized red blood cells) adsorption, affinity chromatography on Sepharose-4B and gel-filtration on SephadexG-200. By SDS-PAGE, purified MPL yielded three bands, with a total molecular mass of 84 ku. By gel-filtration, the molecular mass of MPL was determined to be 86 ku. MPL exhibits high affinity towards D-galactose. The hemagglutination activity of MPL was independent of Ca2+, heat liable and stable in the range of pH 6similar to9. Antibacterial activity of MPL has been observed against Escherichia coli, Bacillus subtilis and Salmonella typhi.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2003年第3期442-446,共5页
Progress In Biochemistry and Biophysics
基金
天津市自然科学基金重点项目 ( 0 0 380 3911)~~
关键词
家蝇蛹凝集素
分离工艺
性质
抑菌活性
血凝活力
糖专一性
固定化红细胞
Musca domestic pupae
lectin
antibacterial activity
haemagglutination activity
carbohydrate specificity
glutaraldehydized erythrocyte
