摘要
采用缓冲液浸提、亲和层析、超滤和离子交换4种方法,从家蝇蛹中分离纯化出一种D–半乳糖特异性结合的凝集素(MPL-1),该组分具有明显的凝血活性和免疫调节活性.聚丙烯酰胺凝胶电泳和高效液相色谱分析结果表明,MPL-1为单一成分的蛋白,其分子质量为80 ku.中性红吞噬实验结果显示,此种凝集素能提高巨噬细胞的吞噬活性,其作用效果具有时间和浓度依赖性.扫描电镜(SEM)观察结果表明,MPL-1作用后的巨噬细胞呈现明显的活化状态,证明MPL-1对巨噬细胞具有明显的免疫调节作用.
A D-galactose specific lectin was purified from Musca domestica pupae （MPL-1）by crude extract in buffer, affinity chromatography,ultra-filtration and ion exchange process, subsequently, lts macrophage immunomodulatory activity in vitro was studied. Detection of PAGE and HPLC show that MPL-1 is homogeneous with the molecular weight of 80 ku. Pinocytic activity of macrophage can be obvious enhanced by MPL-1 in a time and dose dependent manner,and the macrophages are shown to be activated by observation of the microstructures of cells scanning electron microscope （SEM）.
出处
《天津科技大学学报》
CAS
2010年第3期5-8,共4页
Journal of Tianjin University of Science & Technology
基金
国家自然科学基金资助项目(20676103)
国家高技术研究计划(863计划)基金资助项目(2007AA10Z319)
关键词
家蝇蛹
凝集素
纯化
巨噬细胞
免疫调节
Musca domestica pupae
lectin
purification
immunomodulator
macrophage
