摘要
通过适当方法处理寄主并长期保存,是大量繁殖寄生蜂的重要途径.蝇蛹金小蜂是多种有害蝇类的蛹期重要寄生蜂,在生物防治上具有重要价值.本文探讨了蝇蛹金小蜂对-20℃冷冻、6℃冷藏和二氧化碳窒息处理1、3和30d的家蝇蛹以及热处死和热处死后冷藏保存30d家蝇蛹的寄生能力.结果表明:蝇蛹金小蜂可以利用上述蝇蛹,且其后代在胫节长度上均与源自新鲜蛹的寄生蜂胫节长度无显著差异;但除冷冻方法外,寄生蜂后代产量均随寄主保存时间的延长而降低.在保存30d的前提下,冷冻方法保存的寄主上寄生蜂后代最多.表明在大量繁殖蝇蛹金小蜂时,可以利用冷冻等方法对寄主进行处理并保存.
It is treat the wasps' an important way to massively rear parasitoid wasps by using appropriate methods to hosts and preserve them for a long duration. Pachycrepoideus vindemmiae Rondani is a pupal parasitoid of several dipteral pests, being of significance for the biological control of the pests. In this paper, housefly pupae were frozen at -20℃, cold storage-preserved at 6 ℃, and COs-asphyxiated for 1-, 3-, and 30 days, respectively, and some pupae were heat-killed and cold storage-preserved for 30 days, aimed to approach the effects of these treatment methods on the re- production of P. vindemmiae on the pupae. The on the pupae treated with the above-mentioned resuhs showed that P. vindemmiae could reproduce methods, and the tibia length of the offspring had less difference with that on the fresh pupae. However, the reproduction of P. vindemmiae on the pupae treated with the above-mentioned methods except frozen decreased with the increasing preser- ving duration of the pupae. At the prerequisite of preserving for 30 days, frozen pupae had the high- est P. vindemmiae offspring reproduction, suggesting that P. vindemmiae could be massively reared when the housefly pupae were treated by frozen and cold storage-preserved.
出处
《应用生态学报》
CAS
CSCD
北大核心
2013年第3期795-800,共6页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31172145)
教育部高等学校博士学科点科研专项(20113424120005)
安徽省教育厅自然科学基金项目(KJ2011A133)
宁波市重点科技攻关择优委托项目(2009C10004)
重要生物资源保护和利用研究安徽省重点实验室基金项目
大学生创新性实验计划项目(cxsy11009
201210370126)资助
关键词
蝇蛹金小蜂
家蝇蛹
冷冻
二氧化碳
寄主保存
生物防治
Pachycrepoideus vindemmiae
housefly pupae
frozen
carbon dioxide (CO2 )
hostpreservation
biological control.