摘要
目的研究康复训练对大鼠脑梗死灶周围脑源性神经营养因子 (BDNF)表达的影响。方法 60只SD大鼠采用光化学诱导法制作脑梗死大鼠模型 ,于 2 4h后将模型大鼠随机分为康复训练组和制动组。前者每天进行水迷宫、转棒和滚笼训练 ,后者置于筒状网笼内限制活动。分别在 1d、3d、7d、10d、14d取脑行免疫组化染色 ,每组每个时间点各 6只 ,观察各时间点脑梗死灶周围BD NF的表达。结果 1d两组梗死灶周围BDNF阳性神经元均明显增多 ;3d训练组较制动组有更多BDNF阳性神经元 (P <0 0 1) ;随时间延长 ,BDNF阳性神经元减少 ,染色亦变浅 ,在 7d两组梗死灶周围仅有少量BDNF阳性神经元 ;10d、14d两组梗死灶周围偶见BDNF阳性神经元 ;BDNF阳性胶质细胞在 3d、7d、10d、14d大量表达 ,且康复训练组较制动组多 (P <0 0 1)。结论康复训练能促进中枢神经系统表达较高水平的BDNF。
ObjectiveTo study the effects of rehabilitation training on the expression of brain derived neurotrophic factor(BDNF) around the cerebral infarcted area of rats.Methods60 SD rats were randomly divided into rehabilitation group and immobilization group 24 hours after cerebral infarction.The rehabilitation group were given water maze training,rotating bar and rolling cage exercises everyday while the immobilization group were fixed in cages. Histochemistry was used to detect the BDNF expression around the cerebral infarcted area at the 1st,3rd,7th,10th and 14th day after infarction respectively.ResultsAt 1st day after the infarction, the expression of BDNF increased obviously around the infarcted area in both group. More BDNF positive neurons were found in the rehabilitation group than that in the immobilization group at 3rd day ( P <0 01). BDNF positive astrocytes showed obvious increasing in both group. BDNF positive neurons decreased with time going, and the color became lighter at the same time .At the 7th day after infarction, there were only a few positive neurons, and seldom obvious positive neurons could be seen around the infarcted area at 10th and 14th day. Great deal of BDNF positive astrocytes were found at 3rd,7th,10th and 14th day, and the rehabilitation group showed more expression than that of the immobilization group( P <0 01).ConclusionsThe rehabilitation training may increase the expression of BDNF which might take an active part in the recovery of the central nervous system injury and the rebuilding of its function.
出处
《中国康复理论与实践》
CSCD
2003年第3期139-141,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
军队留学人员回国启动基金资助 (97卫科训字 1 1 6号 )
关键词
康复
脑梗死
脑源性神经营养因子
大鼠
rehabilitation
cerebral infarction
brain derived neurotrophic factor(BDNF)
rats
