摘要
目的 :研究实验性黄韧带骨化的超微结构演变特点。材料和方法 :将重组人骨形态发生蛋白 2 (rhBMP 2 )诱发的骨化过程不同时期的动物黄韧带组织行超薄切片 ,用电镜进行观察。结果 :rhBMP 2植入 2周 ,黄韧带内胶原纤维增生 ,弹力纤维减少、断裂 ,成纤维细胞代谢旺盛 ,其间可见散在的不成熟软骨细胞、2种细胞胞浆中均可见大量的粗面内质网和线粒体 ;4周 :黄韧带内软骨细胞明显增多、分化成熟 ,胞浆内可见丰富的粗面内质网和核糖体、线粒体 ,靠近细胞分泌端的基质陷窝中可见数量不等的电子致密小体 ,基质中胶原纤维大量增生 ;6周 :成熟的软骨细胞体积增大 ,呈圆形 ,胞浆内富含线粒体 ,并可见线粒体与细胞膜融合形成分泌小泡 ,细胞周围基质中可见大量的电子致密小体 ,尚可见数量不等的新生弹力纤维 ;8周 :肥大的软骨细胞退变、坏死 ,表现为细胞核皱缩 ,细胞膜及核膜广泛碎裂 ,残留物附近出现数量更多的电子致密小体 ;尚可观察到幼稚骨细胞和骨细胞的存在。结论
Objective:To study the ultrastructure changes du ring the course of experimental ossification of ligamentum flavum.Methods:The li gamentum flavum tissues during ossification induced by recombined human bone mor phogenetic protein-2(rhBMP-2) were made into slice by ultramicrotomy,and obser ved under electric microscope.Results:After implantation of rhBMP-2,microstruct ure changes in 2 weeks are collagen fiber proliferation,elastic fiber decreasing and broken,active metabolism of fibroblast and scattering immature chondrocytes .A great amount of rough endoplasmic reticulum and chondrosome can be seen in th e cytoplasm of fibroblasts and chondrocytes.Changes in 4 weeks are increasing of chondrocytes which differentiate to mature,affluent rough endoplasmic reticulum ,ribosome and chondrosome,different amount of electronic pyknotic bodies in the matrix lacuna on the excreting side of the cells,proliferation of collagen fiber in matrix.Changes in 6 weeks are enlargement of round mature chondrocytes conta ining a great deal of chondrosome in cytoplasm,excreting alveolus formation with fusion of chondrosome and cell membranes,a great amount of electronic pyknotic bodies in the matrix around the cells,different amount of regenerated elastic fi bers.Changes in 8 weeks are degeneration and necrosis of hypertrophic chondrocyt es manifested as shrinking of nucleus and extensive fragmentation of cell membra nes and nuclear membranes,more electronic pyknotic bodies near the residuals.Ost eocytes can also be observed.Conclusion:Chondrocytes in the ligamentum flavum du ring ossification formation are originated from fibroblasts.
出处
《中国矫形外科杂志》
CAS
CSCD
2002年第13期1314-1316,共3页
Orthopedic Journal of China
