摘要
背景:破坏颈椎后方稳定结构会导致黄韧带退变加速,颈椎前方不稳是否会导致后方相应黄韧带及邻近节段黄韧带退变加速还不清楚。目的:观察颈椎不稳动物模型中黄韧带的组织病理学变化及对转化生长因子β1表达的影响。方法:将36只新西兰大白兔随机等分为实验组及对照组。实验组通过颈椎前路穿刺破坏纤维环及抽吸C4/5髓核组织建立兔颈椎不稳动物模型,对照组不做任何处理。结果与结论:与对照组相比,实验组兔C3/4,C4/5,C5/6黄韧带中纤维排列紊乱,玻璃样变性,黄韧带中转化生长因子β1阳性染色面积增加,其中以C4/5最为显著;4,8及12周时2组大白兔C3/4,C4/5,C5/6黄韧带中转化生长因子β1的阳性面积接近。说明颈椎前方不稳会诱发后方黄韧带退变加速,其中以损伤节段黄韧带退变最为严重。
BACKGROUND：Destroying posterior stable structure of cervical vertebra may facilitate the ligamentum flavum regeneration. Whether anterior cervical instability can induce the regeneration in posterior and adjacent ligamentum flavum remains unclear. OBJECTIVE：To observe the changes of transforming growth factor-β1 expression and histopathology in the ligamentum flavum of cervical instability animal models. METHODS：Thirty-six New Zealand white rabbits were randomly divided into control group and experimental group, with 18 rabbits in each group. In the experimental group, cervical instability animal models were established made through destroying annulus fibrosus by anterior puncture and absorbing nucleus pulposus in C4/5 . And no intervention was given to the control group. RESULTS AND CONCLUSION：Compared with the control group, fibers in the C 3/4, 4/5, 5/6 ligamentum flavum arranged disorderly and the glass like degeneration was found in the experimental group. The expression of transforming growth factor-β1 in ligamentum flavum was increased, especial y in C 4/5 , in the experimental group. At 4, 8, 12 weeks, transforming growth factor-β1 expression in the C 3/4, 4/5, 5/6 ligamentum flavum segments was similar between the two groups. Experimental findings indicate that, anterior cervical instability can induce the regeneration in posterior ligamentum flavum, especial y in the injured segment.
出处
《中国组织工程研究》
CAS
北大核心
2015年第18期2891-2895,共5页
Chinese Journal of Tissue Engineering Research
基金
济宁医学院青年基金(20090511)
山东省自然科学青年基金(ZR2014CQ042)~~
