摘要
通过参加2022年中国检验检疫科学研究院组织的ACAS-PT1343沙门菌(Salmonella)、单增李斯特菌(Listeria monocytogenes)检测能力验证计划,分别采用国标法、全自动酶联免疫荧光分析仪法和实时荧光PCR检测法对随机人工污染样品22-A419、22-F730沙门菌检验及22-D378、22-G483单增李斯特菌检验。结果显示,22-A419和22-F730检出沙门菌,血清分型为O(9,12)H(m),确定为肠炎沙门菌;22-D378检出单增李斯特菌,22-G483未检出。采用国标法检测沙门菌和单增李斯特菌,辅以分子生物学方法,不仅降低了假阳性率,也缩短了检测时间。后两种方法可作为大量样品的前筛查及应急检验,对筛查中出现的阳性样本用国标法进行确认,提高了检验检测效率。
Objective To participating in the 2022 ACAS-PT1343 Salmonella and Listeria monocytogenes detection capability validation program organized by the Chinese Academy of Inspection and Quarantine Sciences,three rapid isolation and identification methods for Salmonella and Listeria monocytogenes were used to improve the efficiency of inspection and detection compared with national standard methods.Methods The national standard method,automatic enzyme-linked immunofluorescence analyzer and real-time PCR were used to detect Salmonella 22-A419,22-F730 and Listeria monocytogenes monocytogenes 22-D378,22-G483 in randomly artificilly contaminated samples.Results Salmonella was detected in 22-A419 and 22-F730,and the serum typing was O(9,12)H(m),indicating Salmonella enteritidis;Listeria monocytogenes monocytogenes was detected from 22-D378,but not from 22-G483.Conclusion The detection of Salmonella and Listeria monocytogenes adopts the national standard method,supplemented by the automatic enzyme-linked immunofluorescence analyzer and real-time fluorescent quantitative PCR,which not only reduces the false positive rate,but also shortens the detection time.The latter two methods can be used as pre screening and emergency testing of Large number of samples.Positive samples in screening are confirmed by national standard method,which improves the efficiency of testing.
作者
沈锐
余米
严海元
肖陈
赵壮志
Shen Rui;Yu Mi;Yan Haiyuan;Xiao Chen;Zhao Zhuangzhi(Chongqing Institute for Food and Drug Control,401121;Chongqing Institute of Medicinal Plant Cultivation,408435)
出处
《粮食储藏》
2024年第4期46-51,共6页
Grain Storage
基金
重庆市永川区自然科学基金重点项目(2022yc-jckx20022、2023yc-jckx20004)
重庆市自然科学基金面上项目(CSTB2023NSCQ-MSX1083)
重庆市药品监督管理局、重庆市科学技术局药品科研项目(CQYJKJ2024-01)。
