摘要
目的探究糜烂溃疡性口腔黏膜病患者治疗前后口腔菌群、龈沟液炎症因子和免疫功能变化,并分析口腔菌群与龈沟液炎症因子、免疫功能的关系。方法选取2020年10月至2023年10月我院收治的80例糜烂溃疡性口腔黏膜病患者为研究对象。所有患者均采用曲安奈德联合重组人表皮生长因子治疗,比较治疗前后患者口腔菌群、龈沟液炎症因子[肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)、白细胞介素-6(IL-6)]、免疫功能(CD4^(+)、CD8^(+)、NK细胞)的变化,并采用Pearson相关分析法分析糜烂溃疡性口腔黏膜病患者口腔菌群与龈沟液炎症因子及免疫功能的关系。结果与治疗前相比,治疗后患者TNF-α、CRP、IL-6水平均降低(P<0.05)。与治疗前相比,治疗后患者CD4+细胞比例升高,CD8+及NK细胞比例均降低(均P<0.05)。在属水平上,患者治疗前后口腔中链球菌属(Streptococcus)、奈瑟菌属(Neisseria)及韦荣菌属(Veillonella)的丰度差异均有统计学意义(均P<0.05)。在门水平上,患者治疗后口腔中厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)丰度均显著上升,拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)、梭菌门(Fusobacteria)丰度均显著下降(均P<0.05)。Alpha多样性分析显示,治疗后患者口腔菌群Chao1指数、Observed species指数、Shannon指数、Simpson指数均较治疗前升高(均P<0.05)。Pearson相关性分析显示,糜烂溃疡性口腔黏膜病患者Streptococcus、Neisseria、Veillonella、Firmicutes、Actinobacteria、Chao1指数、Observed species指数、Shannon指数及Simpson指数与TNF-α、CRP、IL-6、CD8+、NK细胞均呈负相关,与CD4^(+)细胞呈正相关(均P<0.05);Bacteroidetes、Proteobacteria、Fusobacteria与TNF-α、CRP、IL-6、CD8^(+)、NK细胞均呈正相关,与CD4^(+)细胞呈负相关(均P<0.05)。结论经过治疗,糜烂溃疡性口腔黏膜病患者口腔菌群结构发生改变,龈沟液炎症因子水平下降,免疫功能增强。�
Objective To explore the changes and relationship among oral flora, gingival crevicular fluid inflammatory factors and immune function before and after treatment of patients with erosive ulcerative oral mucosal disease. Methods A total of 80 patients with erosive ulcerative oral mucosa treated in our hospital from October 2020 to October 2023 were selected as the study objects. All patients were treated with Triamcinolonede combined with recombinant human epidermal growth factor. The changes of oral microflora, gingival sulcus fluid inflammatory factors [tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), interleukin-6 (IL-6)], and immune function [CD4^(+), CD8^(+), NK cells] before and after treatment were compared. Pearson correlation analysis was used to analyze the relationship between oral flora and gingival crevicular fluid inflammatory factors and immune function in patients with erosive ulcerative oral mucosa. Results Compared with before treatment, the levels of TNF-α, CRP and IL-6 were decreased after treatment (all P<0.05). Compared with before treatment, CD4^(+) cells increased, CD8^(+) and NK cells decreased after treatment (all P<0.05). At the genus level, there was a statistically significant difference in the abundance of Streptococcus, Neisseria, and Veillonella before and after treatment (all P<0.05). At the phylum level, after treatment, the abundance of Firmicutes and Actinobacteria significantly increased, while the abundance of Bacteroidetes, Proteobacteria, and Fusobacteria significantly decreased (all P<0.05). The Alpha diversity analysis showed that the Chao1 index, Observed species index, Shannon index and Simpson index were all higher after treatment than before treatment (all P<0.05). Pearson correlation analysis showed that Streptococcus, Neisseria, Veillonella, Firmicutes, Actinobacteria, Chao1 index, Observed species index in patients with erosive ulcerative oral mucosa were negatively correlated with TNF-α, CRP, IL-6, CD8^(+) cells and NK cells, but positively correlated
作者
黄惠
曾翔宇
张莅琼
HUANG Hui;ZENG Xiangyu;ZHANG Liqiong(Department of Stomatology,Mianyang Fulin Hospital,Mianyang,Sichuan 621000,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
北大核心
2024年第4期431-436,共6页
Chinese Journal of Microecology
关键词
糜烂溃疡性口腔黏膜病
口腔菌群
龈沟液炎症因子
免疫功能
Erosive ulcerative oral mucosal disease
Oral flora
Gingival crevicular fluid inflammatory factors
Immune function
