摘要
目的:研究白虎加人参汤对2型糖尿病(T2DM)模型大鼠腺苷一磷酸活化蛋白激酶(AMPK)/固醇反应元件结合蛋白-1(SREBP1)信号通路的影响。方法:通过高脂饲料联合链脲佐菌素(STZ)注射建立T2DM模型大鼠,并用不同剂量的白虎加人参汤灌胃。通过检测各组大鼠造模给药后血清中相关生化指标和肝组织病理学变化,评估白虎加人参汤对T2DM模型大鼠的治疗作用;然后,运用定量聚合酶链反应(qPCR)和蛋白质免疫分析法检测白虎加人参汤干预后,各组大鼠肝组织中AMPK/SREBP1信号通路关键因子腺苷一磷酸活化蛋白激酶(AMPK)、乙酰辅酶A羧化酶1(ACC1)、脂肪酸合成酶(FASN)、ATP柠檬酸裂解酶(ACLY)基因及蛋白水平,初步探究白虎加人参汤治疗T2DM模型大鼠的作用机制。结果:白虎加人参汤可明显降低T2DM模型大鼠血清中空腹血糖(FBG)、口服葡萄糖耐量试验(OGTT)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)、总胆固醇(TC)、三酰甘油(TG)以及低密度脂蛋白胆固醇(LDL-C)水平,升高高密度脂蛋白胆固醇(HDL-C)水平,同时改善T2DM模型大鼠肝组织病理学变化。qPCR及蛋白质免疫分析结果显示,白虎加人参汤可显著降低T2DM模型大鼠肝组织中AMPK基因与蛋白水平,上调SREBP1、ACC1、ACLY、FASN基因与蛋白水平。结论:白虎加人参汤对T2DM模型大鼠具有治疗作用,其作用机制可能与调节肝组织中AMPK/SREBP1信号通路,改善脂代谢有关。
Objective:To investigate the effect of Baihu Decoction with Ginseng on AMP-activated protein kinase/sterol regulatory element-binding protein-1(AMPK/SREBP1)signaling pathway in type 2 diabetes mellitus(T2DM)model rats.Methods:T2DM model rats were established using high-fat feed combined with streptozotocin(STZ)injection,followed by intragastrically administrating different doses of Baihu Decoction with Ginseng.The therapeutic effect of Baihu Decoction with Ginseng on T2DM model rats was evaluated by detecting the relevant serum biochemical indices and liver histopathological changes after intragastrical administration in each group of rats;Then,after the Baihu Decoction with Ginseng intervention was detected by qPCR and Western blot method,the genes and protein levels of key factors AMPLK/SREBP1 signaling pathway,such as AMPK,acetyl-coenzyme A carboxylase 1(ACC1),fatty acid synthase(FASN),and ATP citrate lyase(ACLY)were detected in the liver tissues of rats in each group,and the mechanism of action of Baihu Decoction with Ginseng in the treatment of T2DM model rats was preliminarily explored.Results:Baihu Decoction with Ginseng significantly reduced the levels of fasting blood glucose(FBG),oral glucose tolerance test(OGTT),glutamate-pyruvate transaminase(GPT),glutamic-oxaloacetic transaminase(GOT),total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)in the serum of T2DM models,and increased the levels of high-density lipoprotein cholesterol(HDL-C).It also improved liver histopathological changes in the liver of T2DM model rats.The qPCR and Western blot results showed that Baihu Decoction with Ginseng could significantly reduce the levels of AMPK gene and protein levels in the liver tissue of T2DM model rats,and upregulate the genes and proteins levels of SREBP1,ACC1,ACLY,FASN.Conclusion:Baihu Decoction with Ginseng has a therapeutic effect on T2DM model rats,possibly by modulating the AMPK/SREBP1 signaling pathway in liver tissue and improving lipid metabolism.
作者
吕树泉
张辉
张淑芳
潘保朝
姚彬
苏秀海
王元松
李文东
李函舟
田甜
苗静
LYU Shuquan;ZHANG Hui;ZHANG Shufang;PAN Baochao;YAO Bin;SU Xiuhai;WANG Yuansong;LI Wendong;LI Hanzhou;TIAN Tian;MIAO Jing(Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Hebei College of Traditional Chinese Medicine,Cangzhou 061001,China;Graduate School of Hebei College of Traditional Chinese Medicine,Shijiazhuang 050091,China;Graduate School of Chengde Medical College,Chengde 067000,China;Tianjin Second People′s Hospital,Tianjin 300192,China)
出处
《世界中医药》
CAS
北大核心
2024年第7期962-968,共7页
World Chinese Medicine
基金
国家自然科学基金项目(82274424)
沧州市重点研发计划项目(213106042)
苏秀海全国名老中医药专家传承工作室(国中医药人教函〔2022〕75号)。
