摘要
[目的]探讨分析外周血二胺氧化酶(DAO)及高迁移率族蛋白B1(HMGBl)联合检测对腹部手术后24 h肠道黏膜屏障功能障碍的预测价值。[方法]:选取108例腹部手术患者,根据术后24 h是否发生肠道黏膜屏障功能障碍将其分为:肠道黏膜屏障功能障碍组(A组)50例和无肠道黏膜屏障功能障碍组(B组)58例。检测2组患者术后12 h和24 h外周血DAO及HMGBl水平,分析各时间点其指标水平变化与肠道黏膜屏障功能障碍的关系,并评估其对诊断腹部手术后24 h肠道黏膜屏障功能障碍的预测价值。[结果]A组与B组患者术前外周血DAO及HMGB1水平比较差异无统计学意义(P>0.05);术后12 h,2组患者外周血DAO及HMGB1水平较术前显著升高,且A组患者水平更高,差异有统计学意义(P<0.05);术后24 h,2组患者外周血DAO及HMGB1水平较术后12 h降低,但依旧高于术前,差异有统计学意义(P<0.05);外周血DAO及HMGB1联合检测对诊断腹部手术后24 h肠道黏膜屏障功能障碍的敏感度高于单一外周血DAO检测(χ^(2)=4.987,P=0.026)、高于单一HMGB1检测(χ^(2)=12.403,P<0.001);联合检测的特异度高于单一外周血DAO检测(χ^(2)=20.787,P<0.001)、高于单一HMGB1检测(χ^(2)=7.421,P=0.006)、联合检测的准确度高于单一外周血DAO检测(χ^(2)=23.775,P<0.001)、高于单一HMGB1检测(χ^(2)=18.921,P<0.001)。[结论]外周血DAO及HMGB1联合检测对腹部手术后24 h肠道黏膜屏障功能障碍具有较好预测价值,敏感度和特异度高,可为腹部手术后24 h肠道黏膜屏障功能障碍诊断提供指导依据,具有较高的临床应用价值。
[Objective]To explore and analyze the predictive value of combined detection of peripheral blood diamine oxidase(DAO)and high mobility group protein B1(HMGB1)for intestinal mucosal barrier dysfunction 24 h after abdominal surgery.[Methods]Total of 108 patients with abdominal operation were selected,and divided them into the intestinal mucosal barrier dysfunction group with 50 cases and no intestinal mucosal barrier with 58 cases according to whether they had intestinal mucosal barrier dysfunction 24 h after the operation in the mucosal barrier dysfunction group.The levels of diamine oxidase(DAO)and high-mobility group box 1(HMGBl)in the peripheral blood of the two groups of patients were detected at 12 h and 24 h after surgery,and the relationship between the changes in the index levels at each time point and the intestinal mucosal barrier dysfunction was analyzed.To evaluate its predictive value in diagnosing intestinal mucosal barrier dysfunction 24 h after abdominal surgery.[Results]Before operation,there was no significant difference in the levels of DAO and HMGB1 in the peripheral blood of the two groups of patients(P>0.05);12 h after the operation,the levels of DAO and HMGB1 in the peripheral blood of the two groups were significantly higher than those before the operation,and the intestinal mucosal barrier function.The level of the patients in the disorder group was higher,and the difference reached statistical significance(P<0.05);24 h after the operation,the levels of DAO and HMGB1 in the peripheral blood of the two groups were lower than those at the 12 h after the operation,but they were still higher than those before the operation,and the difference reached statistics.Scientific significance(P<0.05).The sensitivity of the combined detection of peripheral blood DAO and HMGB1 in the diagnosis of intestinal mucosal barrier dysfunction at 24 h after abdominal surgery was higher than that of a single peripheral blood DAO test(χ~2=4.987,P=0.026),and higher than a single HMGB1 test(χ~2=12.403,P<0.001);the
作者
阎海
王尧
鞠雷
YAN Hai;WANG Yao;JU Lei(Panjin Central Hospital Pathology,124000 Panjin,Liaoning,China)
出处
《临床消化病杂志》
CAS
2024年第2期100-104,共5页
Chinese Journal of Clinical Gastroenterology
