摘要
研究中麻黄(Ephedra intermedia Schrenk et C.A.Mey.)干燥草质茎的化学成分及其抗哮喘活性。应用硅胶柱色谱、Sephadex LH-20柱色谱、Toyopreal HW-40C柱色谱以及半制备型高效液相色谱等多种手段对中麻黄草质茎的50%丙酮提取物进行化学成分研究,从中分离鉴定了22个化合物,根据其理化性质和波谱学数据鉴定其结构,分别为4-甲氧基肉桂酸(1)、肉桂酸(2)、ω-hydroxypropioguaiacone(3)、threo-8 S-7-methoxysyringylglycerol(4)、3-(2,4′-dihydroxy-3′,5′-dimethoxyphenyl)propanoic acid(5)、8-hydroxy-9-methyl-7-(4-hydroxy-3,5-dimethoxyphenyl)-7-propanone(6)、3-羟基-1-(4-羟基-3,5-二甲氧基苯基)-1-丙酮(7)、2-羟基-1-(4-羟基-3-甲氧基)苯基-1-丙酮(8)、异咖啡酸甲酯(9)、trans-syringin(10)、cis-syringin(11)、(4 R)-4-hydroxy-4-(2-hydroxypropan-2-yl)cyclo-hex-1-ene-1-carboxylic acid(12)、7-羟基-α-松油醇(13)、去氢吐叶醇(14)、脱落酸(15)、黑麦草素内脂(16)、(E)-3-(3-hydroxybut-1-enyl)-2,4,4-trimethylcyclohexa-2,5-dienone(17)、3-(3′-hydroxybutyl)-2,4,4-trimethylcyclohexa-2,5-dienone(18)、对异丙基苯甲酸(19)、p-cymen-7-ylβ-D-glucopyranoside(20)、acyclic 7-hydroxygeranic acid(21)、7,7-dimethylbicyclo[3.1.1]hept-2-ene-1-carboxylic acid(22),其中化合物3~6、8、10~14、17~19、21、22为首次从该植物中分离得到,同时经体外抗哮喘活性筛选,结果显示化合物11和14能够显著抑制β-氨基己糖苷酶的释放。
To study the chemical constituents of the herbaceous stems of Ephedra intermedia Schrenk et C.A.Mey.Twenty-two compounds were isolated from the 50%aqueous acetone extract of Ephedra intermedia by silica gel,sephadex LH-20 column chromatography,Toyopreal HW-40C column chromatography,as well as semi-preparative HPLC methods.Their structures were identified by spectral analysis as 4-methoxycinnamic acid(1),cinnamic acid(2),ω-hydroxypropioguaiacone(3),threo-8 S-7-methoxysyringylglycerol(4),3-(2,4′-dihydroxy-3′,5′-dimethoxyphenyl)propanoic acid(5),8-hydroxy-9-methyl-7-(4-hydroxy-3,5-dimethoxyphenyl)-7-propanone(6),3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(7),2-hydroxyl-1-[4-hydroxy-3-methoxyphenyl(propan-1-one)](8),methyl caffeate(9),trans-syringin(10),cis-syringin(11),(4 R)-4-hydroxy-4-(2-hydroxypropan-2-yl)cyclo-hex-1-ene-1-carboxylic acid(12),7-hydroxyterpineol(13),dehydrovomifoliol(14),abscisic acid(15),loliolide(16),(E)-3-(3-hydroxybut-1-enyl)-2,4,4-trimethylcyclohexa-2,5-dienone(17),3-(3′-hydroxybutyl)-2,4,4-trimethylcyclohexa-2,5-dienone(18),4-isopropyl benzoic acid(19),p-cymen-7-ylβ-D-glucopyranoside(20),acyclic 7-hydroxygeranic acid(21),7,7-dimethylbicyclo[3.1.1]hept-2-ene-1-carboxylic acid(22).And compounds 3-6,8,10-14,17-19,21 and 22 are isolated from this plant for the first time.The results of their anti-asthma activities showed that compounds 11,14 could significantly inhibit the release ofβ-aminohexosidase.
作者
范锡玲
曹彦刚
焦新棉
郑晓珂
冯卫生
FAN Xi-ling;CAO Yan-gang;JIAO Xin-mian;ZHENG Xiao-ke;FENG Wei-sheng(The Engineering and Technology Center for Chinese Medicine Development of Henan Province,School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第3期415-422,484,共9页
Natural Product Research and Development
基金
国家重点研发计划-中医药现代化研究重点专项(2019 YFC1708800)。
关键词
麻黄属
中麻黄
结构鉴定
抗哮喘活性
Ephedra
Ephedra intermedia
structural identification
anti-asthmatic activities
