摘要
为筛选苹果轮纹病抗性基因,以苹果轮纹病抗、感株系为材料,白藜芦醇处理后针刺法接种轮纹病菌,利用高通量测序技术对接菌0 h和96 h的样品进行差异表达基因分析。试验共构建了24个文库,转录组测序共检测到173.80 Gb Clean Data,各样品Clean Data均达到6.05 Gb,Q30值在90.67%及以上。差异表达基因(DEGs)分析结果表明,白藜芦醇处理后抗病材料的DEGs多于感病材料,接菌96 h的DEGs多于接菌0 h的。KEGG代谢通路富集分析表明,抗病材料富集的通路远远多于感病材料,且富集到抗病相关通路如植物激素信号转导和植物-病原互作的基因也多于感病材料;此外,抗病材料还富集到与木质素合成相关的苯丙素生物合成途径以及类黄酮生物合成通路。可见,在白藜芦醇处理后接菌,抗病材料叶片内的防御机制变化更丰富,可以更好地应对轮纹病菌的侵染。本研究结果可为了解白藜芦醇影响苹果轮纹病抗性的分子机制以及选育苹果抗轮纹病新品种提供理论依据。
This study aimed to screen the resistance genes of apple ring rot.After resveratrol treatment,the resistant and susceptible materials were inoculated with the pathogen of apple ring rot by acupuncture method.The samples after inoculation for 0 h and 96 h were used for differential expression gene(DEG) analysis by high-throughput sequencing technology.A total of 24 libraries were constructed.A total of 173.80 Gb Clean Data were detected by transcriptome sequencing.The Clean Data of each sample reached 6.05 Gb,and the Q30 value was 90.67% and above.The DEG analysis results showed that the DEGs of resistant materials after resveratrol treatment were more than those of susceptible materials,and the DEGs at 96 h after inoculation were more than those at 0 h after inoculation.KEGG metabolic pathway enrichment analysis showed that the enriched pathways of disease-resistant materials were far more than those of susceptible materials,and the genes enriched in disease-resistance related pathways such as plant hormone signal transduction and plant-pathogen interaction were also more than those of susceptible materials.In addition,the DEGs of resistant materials were also enriched in phenylpropanoid biosynthesis pathway and flavonoid biosynthesis pathway related to lignin synthesis.It could be seen that after resveratrol treatment,the defense mechanism in the leaves of disease-resistant materials changed more abundant,so as to better cope with the infection of Botryosphaeria dothidea.The results of this study couldprovide theoretical references for understanding the molecular mechanism of resveratrol affecting apple ring rot resistance and breeding new apple varieties with ring rot resistance.
作者
张洋红
李爽
陈昊伟
李佳
徐继忠
Zhang Yanghong;Li Shuang;Chen Haowei;Li Jia;Xu Jizhong(College of Horticulture,Hebei Agricultural University,Baoding 071000,China)
出处
《山东农业科学》
北大核心
2024年第2期14-22,共9页
Shandong Agricultural Sciences
基金
现代农业产业技术体系建设专项(CARS-27)
河北省现代农业产业技术体系苹果创新团队项目(HBCT2021100210)
河北省重点研发计划项目(21326308D-02-03)。
关键词
苹果轮纹病
白藜芦醇
转录组
差异表达基因
Apple ring rot
Resveratrol
Transcriptome
Differential expression gene
