摘要
以黄秋葵籽为原料,对酶法萃取黄秋葵籽油的最优工艺进行探索研究并研究其抗氧化性。首先,通过对酶制剂的筛选,选用纤维素酶+酸性蛋白酶(1∶1)复合酶作为水解酶。再结合单因素实验探索不同因素对黄秋葵籽油提取率的影响。在此基础上,采用Box-Behnken响应面优化设计三因素三水平试验,根据回归分析得出水酶法萃取黄秋葵籽油的最佳条件为:酶解温度55℃、酶解pH为4.0、料液比1∶4、酶添加量为4.25%、酶解时间为3.25 h。在此条件下,黄秋葵籽油的提取率为(56.56±0.58)%。抗氧化能力测试结果表明:秋葵籽油对DPPH自由基和ABTS自由基均有一定的清除能力,其中当秋葵籽油浓度为50 mg/mL时,对DPPH自由基的清除能力可达到95.25%,与相同浓度下,VC对DPPH自由基清除能力接近。该研究为黄秋葵籽油的水酶法提取工艺提供了理论支持和技术依据,为黄秋葵籽资源的综合利用提供参考。
This study aimed to explore and optimize the enzymatic extraction process of okra seed oil using cellulase and acid protease(l:l)as the hydrolytic enzyme,and investigate its antioxidant properties.Firstly,enzyme preparations were screened,and the cellulase and acid protease composite enzyme was selected for hydrolysis.Then,the effects of different factors on the extraction yield of okra seed oil were explored through single-factor experiments.Based on this,a Box-Behnken response surface design with three factors and three levels was employed to optimize the enzymatic extraction process.The optimal conditions for the hydrolysis of okra seed oil were determined as follows:hydrolysis temperature of 55°C,hydrolysis pH of 4.0,solidto-liquid ratio of 1:4,enzyme dosage of 4.25%,and hydrolysis time of 3.25 hours.Under these conditions,the extraction yield of okra seed oil was(56.56±0.58)%.The antioxidant capacity test results revealed that okra seed oil exhibited significant scavenging abilities against DPPH and ABTS free radicals.Particularly,at a concentration of 50 mg/mL,the clearance rate of DPPH free radicals reached 95.25%,which was comparable to the clearance capacity of VC(Vitamin C)at the same concentration.This study provides theoretical support and technical basis for the enzymatic extraction process of okra seed oil and offers references for the comprehensive utilization of okra seed resources.
作者
李双芳
夏琳
刘雨辰
吴博雄
刘生杰
季春艳
LI Shuangfang;XIA Lin;LIU Yuchen;WU Boxiong;LIU Shengjie;JI Chunyan(College of Information Engineering,Fuyang Normal University,Fuyang Anhui 236041,China;School of Biological Science and Food Engineering,Fuyang Normal University,Fuyang,Anhui 236037,China)
出处
《阜阳师范大学学报（自然科学版）》
2023年第4期33-41,共9页
Journal of Fuyang Normal University:Natural Science
基金
安徽省高校自然科学重点项目(2023AH052852)
安徽省大学生创新创业训练计划(S20212361911,S202213619014)
阜阳地方农产品食品工艺开发科研创新团队(FX2020KCT02)。
关键词
黄秋葵籽油
复合酶法
响应面优化法
抗氧化性
okra seed oil
water enzymatic method
response surface optimization method
oxidize resistance
