摘要
目的分析肠道巨单胞菌属(Megamonas)、巴恩斯菌属(Barnesiella)、梭菌属(Clostridium)和瘤胃球菌属(Ruminococcus)在新发2型糖尿病(T2DM)、糖调节受损(IGR)与糖耐量正常(NGT)人群的丰度差异,以及菌属与膳食摄入量的相关性。方法于2021年9月至2022年6月选取新疆医科大学第一附属医院和新疆医科大学第五附属医院72例新发T2DM、35例IGR及124例NGT为研究对象,构建质粒制作菌属标准品并绘制标准曲线,采用实时荧光定量PCR(qPCR)检测4种菌属丰度并比较其差异。采取食物频率问卷(FFQ)调查研究对象的膳食情况,分析4种菌属与膳食摄入量的相关性。结果Megamonas、Barnesiella、Clostridium、Ruminococcus丰度在3组间比较,差异均有统计学意义(P<0.05);T2DM组Barnesiella、Clostridium、Ruminococcus丰度较NGT组和IGR组降低;且T2DM组Megamonas和Barnesiella丰度明显低于IGR组(P<0.05)。3组蔬菜、水果和蛋类的摄入量比较,差异均有统计学意义(P<0.05)。Barnesiella的菌群丰度对数值(lgSQ值)与平均每人每日油、平均每人每日动物油摄入量呈正相关(r=0.187、0.208,P<0.05);Clostridium的lgSQ值与空腹血糖(FPG)、总胆固醇、甘油三酯、平均每人每日盐摄入量呈负相关(r=-0.280、-0.165、-0.255、-0.167,P<0.05);Ruminococcus的lgSQ值与FPG、高密度脂蛋白胆固醇、平均每人每日盐摄入量呈负相关(r=-0.213、-0.131、-0.182,P<0.05),与水果摄入量呈正相关(r=0.131,P<0.05)。结论IGR和T2DM患者肠道Megamonas、Barnesiella、Clostridium和Ruminococcus丰度发生改变,且与部分膳食摄入量相关。早期对糖尿病前期IGR进行饮食或肠道菌群调节干预可为T2DM提供治疗靶点和新思路。
Objective To analyze the abundance difference of gut microbiota at genus level such as Megamonas,Barnesiella,Clostridium and Ruminococcus among the patients with newly diagnosed diabetes mellitus type 2(T2DM),the patients with impaired glucose regulation(IGR)and the subjects with normal glucose tolerance(NGT),and the correlation between the abundance of bacterial genus and dietary intakes.Methods A total of 72 patients with newly diagnosed T2DM,35 patients with IGR and 124 subjects with NGT in the First Affiliated Hospital of Xinjiang Medical University and the Fifth Affiliated Hospital of Xinjiang Medical University from September 2021 to June 2022 were enrolled as the study subjects.The plasmid was constructed for preparing the standard product of bacterial genus and the standard curve was drawn.The abundances of four bacterial genera were detected by using the real time fluorescence quantitative PCR(qPCR),and their differences were compared.The dietary status of the study subjects was investigated by using the food frequency questionnaire(FFQ),and the correlation between the four genera and the dietary intakes was analyzed.Results The abundances of Megamonas,Barnesiella,Clostridium and Ruminococcus had statistically significantly differences among the three groups(P<0.05).The abundances of Barnesiella,Clostridium and Ruminococcus in the T2DM group were decreased compared with those in the NGT and IGR groups,moreover the abundances of Megamonas and Barnesiella in the T2DM group were significantly lower than those in the IGR group(P<0.05).There were statistically significant differences in the intakes of vegetables,fruits and eggs among the three groups(P<0.05).The logarithmic abundance value(lgSQ value)of Barnesiella was positively correlated with the average daily per capita oil intake and the average daily per capita animal oil intake(r=0.187,0.208,P<0.05).The lgSQ value of Clostridium was negatively correlated with fasting blood glucose(FPG),total cholesterol,triglycerides and average daily per capita salt i
作者
薛澳徽
王玲
陈文婷
赵炳尧
张冰
热比亚·努力
XUE Aohui;WANG Ling;CHEN Wenting;ZHAO Bingyao;ZHANG Bing;REBIYA Nuli(Basic Medical College,Xinjiang Medical University,Urumqi,Xinjiang 830017,China;The Fifth Clinical Medical College of Xinjiang Medical University,Urumqi,Xinjiang 830011,China;Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases,Urumqi,Xinjiang 830017,China)
出处
《重庆医学》
CAS
2023年第22期3368-3375,共8页
Chongqing medicine
基金
国家自然科学基金项目(81860743)
新疆维吾尔自治区自然科学基金项目(2019D01C194)。
