摘要
目的 探讨老年甲状腺结节合并糖尿病患者外周血DNA甲基化/羟甲基化的检测及其临床意义。方法 选择2021年1月至2022年12月兰州市第一人民医院收治的136例老年甲状腺结节合并糖尿病患者为研究组,另选同期体检正常的135例老年人为对照组。采集所有入组对象一般资料,测定空腹血糖(FPG)、餐后2 h血糖(2hPBG)、糖化血红蛋白(HbA1c)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离三碘甲状腺原氨酸(FT3)、游离四碘甲状腺原氨酸(FT4)、促甲状腺激素(TSH)、甲状腺球蛋白抗体(TGAb)和甲状腺过氧化物酶抗体(TPOAb)水平。采用酶联免疫吸附法检测外周血全基因组5-甲基胞嘧啶(5-mC)、5羟甲基胞嘧啶(5-hmC)、细胞间黏附分子-1(ICAM-1)和甲基双加氧酶(TET2)水平。采用SPSS 21.0统计软件进行数据分析。根据数据类型,分别采用t检验、Mann-Whitney U检验或χ2检验进行组间比较。采用Spearman相关性分析探讨外周血5-mC、5-hmC与甲状腺结节合并糖尿病的相关性。结果 研究组患者FPG、2hPBG、HbA1c、TSH、TgAb和TPOAb水平均高于对照组,FT3低于对照组,差异均有统计学意义(P<0.05)。研究组外周血5-mC、5-hmC水平均高于对照组[6.64%(3.53%,8.53%)和4.28%(1.70%,7.20%);50.41%(35.00%,63.27%)和36.51%(17.81%,50.06%)];外周血TET2、ICAM-1水平均高于对照组[9.37(5.70,13.56)和4.56(1.52,6.99)pg/ml;4.98(2.35,7.01)和0.97(0.15,1.60)ng/ml],差异均有统计学意义(P<0.001)。Spearman相关性分析结果显示,5-mC与TgAb、TPOAb和HbA1c呈正相关(r=0.413,0.348,0.405;P<0.05);5-hmC与TgAb、TPOAb、FBG和2hPG呈正相关(r=0.375,0.406,0.376,0.311;P<0.05);5-mC与5-hmC呈正相关(r=0.256;P<0.05)。结论 老年甲状腺结节合并糖尿病患者外周血全基因组DNA甲基化与羟甲基化水平显著正常人群,外周血5-mC、5-hmC水平与老年甲状腺结节、糖尿病发病显著相关。
Objective To investigate the expression and clinical significance of DNA methylation/hydroxymethylation in peripheral blood of elderly patients with thyroid nodules and diabetes mellitus.Methods A total of 136 elderly diabetes patients complicated with thyroid nodules admitted to our hospital from January 2021 to December 2022 were recruited as the study group,and another 135 elderly healthy age-matched individuals who took physical examination at the same time served as the control group.Their general data were collected,and serum levels of fasting blood glucose(FBG),2h postprandial blood glucose(2hPBG),glycosylated hemoglobin A1c(HbA1c),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),free triiodothyronine(FT3),free tetraiodothyronine(FT4),thyroid stimulating hormone(TSH),anti-thyroglobulin antibodies(TGAb)and thyroid peroxidase antibody(TPOAb)were detected and recorded.The levels of whole genome 5-methylcytosine(5-mC),5-hydroxy methylcytosine(5-hmC),intercellular cell adhesion molecule-1(ICAM-1)and ten-eleven translocation-2(TET2)in the peripheral blood were measured with enzyme linked immunosorbent assay(ELISA).SPSS statistics 21.0 was used for data analysis.Depending on data type,student′s t test,Mann-Whitney U test or Chi-square test was used for intergroup comparison.Spearman correlation analysis was employed to analyze the relationship between 5-mC and 5-hmC levels in peripheral blood and thyroid nodules with diabetes mellitus.Results The serum levels of FBG,2hPBG,HbA1c,TSH,TgAb and TPOAb were significantly higher,while that of FT3 was obviously lower in the study group than the control group(P<0.05).Higher levels of 5-mC and 5-hmC in peripheral blood were observed in the study group than the control group[6.64%(3.53%,8.53%)vs 4.28%(1.70%,7.20%),50.41%(35.00%,63.27%)vs 36.51%(17.81%,50.06%);P<0.001],and so were the levels of TET2 and ICAM-1[9.37(5.70,13.56)vs 4.56(1.52,6.99)pg/ml,4.98(2.35,7.01)vs 0.97(0.15,1.60)ng/ml;P<0.001].Spearman correlation a
作者
马晓权
方兴中
成建荣
Ma Xiaoquan;Fang Xingzhong;Cheng Jianrong(Department of General Surgery,Lanzhou First People′s Hospital,Lanzhou 730050,China)
出处
《中华老年多器官疾病杂志》
2023年第10期756-760,共5页
Chinese Journal of Multiple Organ Diseases in the Elderly
基金
兰州市科学技术局科技发展计划项目(2022-ZD-7)。
