摘要
目前在灯盏细辛[Erigeron breviscapus(Vant.)Hand.-Mazz.]的组织培养研究中,培养基的激素种类、配比及浓度等方面差异较大,鉴于此,本研究以药用植物灯盏细辛无菌苗叶片作为外植体,研究了不同激素配比及不同浓度激素对灯盏细辛的愈伤组织诱导、不定芽分化及生根培养影响。研究结果表明,当6-BA与NAA联合使用时有利于愈伤组织的增殖,最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,诱导率达到100%;以叶片作为外植体,当分裂素/生长素比例较高时适宜不定芽的分化,最适宜培养基为MS+6-BA 2.0 mg/L+NAA 0.2~0.5 mg/L,培养30 d后可获得无根苗;无根苗的生根培养需在低无机盐培养基上添加低浓度生长素,最适宜培养基为1/2MS+NAA 0.3 mg/L+IBA 0.5 mg/L,生根率为100%。本研究利用植物组织培养技术对灯盏细辛组培体系进行系统研究,可缩短灯盏细辛的繁殖周期,解决药材供求矛盾带来的资源匮乏问题,从而实现中药野生资源的可持续利用。
Currently,there are great differences in the hormone types,proportions and concentrations of the medium in tissue culture studies of Erigeron breviscapus.In view of this,the leaves of sterile seedlings of medicinal plant Erigeron breviscapus were used as explants and the effect of different combinations and different concentrations of hormones on callus induction,adventitious bud induction and root information of Erigeron breviscapus were studied.The results showed that the combination of 6-BA and NAA was beneficial to the proliferation of callus.The optimal medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,and the induction rate reached 100%.With leaves as explants,it was suitable for adventitious buds differentiation when the ratio of cytokinin/auxin was higher.The optimal medium was MS+6-BA 2.0 mg/L+NAA 0.2~0.5 mg/L and the rootless shoots could be obtained after 30 d of cultivation.For rooting induction of rootless seedlings,low concentration auxin should be added to low inorganic salt MS medium.The optimal medium was 1/2MS+NAA 0.3 mg/L+IBA 0.5 mg/L,and the induction rate of root formation was 100%.In this study,plant tissue culture technology was used to study the tissue culture system of E.breviscapus systematically,which could accelerate the reproductive cycle of E.breviscapus,solve the problem of resource shortage caused by the contradiction between supply and demand of medicinal materials,and realize the sustainable utilization of wild resources of traditional Chinese medicine.
作者
赵瑜君
于一凡
黄璐琦
Zhao Yujun;Yu Yifan;Huang Luqi(State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700;School of Food and Biological Engineering,Jiangsu University,Zhenjiang,212013)
出处
《分子植物育种》
CAS
北大核心
2023年第15期5058-5065,共8页
Molecular Plant Breeding
基金
中央本级重大增减支项目(2060302)
中央级公益性科研院所基本科研业务费专项(ZZ15-YQ-061)共同资助。
关键词
灯盏细辛
组织培养
愈伤组织诱导
不定芽分化
生根
Erigeron breviscapus
Tissue culture
Callus induction
Adventitious bud induction
Strike root
