摘要
目的:开发以人参—酸枣仁为原料的相关保健品。方法:应用HPLC、紫外分光光度计对人参、酸枣仁不同配伍比例进行化学成分研究;应用DPPH自由基、ABTS自由基、羟自由基和超氧阴离子自由基清除率分析样品的体外抗氧化活性;同时采用灰色关联度法,以熵权法所得权重为分辨系数进行统计分析,结合性状进行综合评价优选出最佳配伍比例。结果:人参—酸枣仁不同配比的样品的抗氧化活性与总酚酸、酸枣仁皂苷A、斯皮诺素含量呈显著相关关系;m_(人参)∶m_(酸枣仁)为1∶1的样品的总酚酸、酸枣仁皂苷A、斯皮诺素含量高于其他配比的,且其抗氧化活性强于其他配比的,说明抗氧化活性的药效物质基础是总酚酸、酸枣仁皂苷A、斯皮诺素。结论:人参—酸枣仁抗氧化活性最佳配比(m_(人参)∶m_(酸枣仁))为1∶1。
Objective:This study aimed to develop relevant health products with ginseng and semen ziziphi spinosae as raw materials.Methods:HPLC and UV spectrophotometer were used to study the chemical components of different compatibility ratios of ginseng and semen ziziphi spinosae.The antioxidant activity in vitro of ginseng and semen ziziphi spinosae with different compatibility ratios was analyzed by DPPH free radical,ABTS~+free radical,hydroxyl free radical and superoxide anion free radical scavenging rate.Moreover,the grey relational degree method was used,and entropy weight method was used as the discrimination coefficient for statistical analysis,combined with the characteristics of comprehensive evaluation to optimize the compatibility ratio.Results:The correlation analysis showed that the antioxidant activity of ginseng-semen ziziphi spinosae and the content of total phenolic acid,Jujuboside A and spinosin were significantly correlated;and the chemical content determination results showed that the total phenolic acid,Jujuboside A and spinosin content of ginseng-semen ziziphi spinosae(1∶1) were higher than the other ratio.The results showed that its antioxidant activity was stronger than other ratios.Furthermore,the pharmacodynamic bases of its antioxidant activity were total phenolic acid,Jujuboside A,and spinosin.Conclusion:The optimal ratio of antioxidant activity of ginseng and semen ziziphi spinosae is m _(ginseng)∶m_(semen ziziphi spinosae) at 1∶1.
作者
肖凤琴
王欣
刘晖
董红影
周文文
徐志佳
李光哲
严铭铭
XIAO Feng-qin;WANG Xin;LIU Hui;DONG Hong-ying;ZHOU Wen-wen;XUE Zhi-jia;LI Guang-zhe;YAN Ming-ming(Changchun University of Chinese Medicine,Changchun,Jilin 130117,China;Jinlin Provincial Science and Technology Innovation Center of Health Food of Chinese Medicine,Changchun,Jilin 130117,China)
出处
《食品与机械》
CSCD
北大核心
2023年第6期173-179,共7页
Food and Machinery
基金
国家重点研发计划(编号:2017YEC1702100)
吉林省中医药保健食品科技创新中心资助项目(编号:20180623041Tc)
长春中医药大学大学生国家级创新创业训练计划支持项目(编号:202110199030)。
关键词
人参
酸枣仁
配比
化学成分
抗氧化活性
相关性分析
ginseng
semen ziziphi spinosae
ratio
chemical composition
antioxidant activity
correlation analysis
