摘要
研究旨在优化超声波辅助液体双相渗透法(ULDPM)同步提取黄贞菌质总黄酮与多糖的工艺条件,并测定其体外抗氧化活性。结果显示,ULDPM同步提取黄贞菌质总黄酮与多糖的最佳工艺条件为上相液料比11 mL/g、下相液料比15 mL/g、超声功率69 W、超声温度49°C、超声时间55 min。在上述条件下,黄贞菌质总黄酮与多糖的得率分别为142.00μg/g和26.12 mg/g。浓度为2.0 g/L时,黄贞菌质总黄酮对DPPH自由基、ABTS自由基的清除率以及还原力分别为87.3%、80.3%、0.9;黄贞菌质多糖对DPPH自由基、ABTS自由基的清除率以及还原力分别为49.5%、43.3%以及1.2。研究表明,ULDPM可同步提取黄贞菌质总黄酮和多糖,且回归模型具有极显著性,可应用于预测黄贞菌质总黄酮与多糖的得率,且黄贞菌质总黄酮与多糖具有较好的体外抗氧化活性。
The experiment was to study the optimization of simultaneous extraction technology of total flavonoids and polysaccharides from Huang Zhen mycoplasm by ULDPM,and measure their in vitro antioxidant activity.The optimum extraction conditions for simultaneous extraction of total flavonoids and polysaccharides from Huang Zhen mycoplasm by ULDPM were upper phase solid-to-liquid ratio of 11 mL/g,lower phase solid-to-liquid ratio 15 mL/g,ultrasonic power 69 W,ultrasonic temperature 49℃and ultrasonic time 55 min.Under the above conditions,the extraction of Huang Zhen mycoplasm total flavonoids and polysaccharides were 142.00μg/g and 26.12 mg/g,respectively.At a concentration of 2.0 g/L,the clearance of DPPH radical and ABTS radical by total flavonoids were 87.3%,80.3%and 0.9,respectively.The clearance of DPPH radical and ABTS radical by polysaccharide were 49.5%,43.3%and 1.2,respectively.ULDPM can extract total flavonoids and polysaccharide simultaneously,and the regression model can be used to reasonably predict the rate of Huang Zhen mycoplasm total flavonoids and polysaccharides.The total flavonoids and polysaccharides from Huang Zhen mycoplasm have good antioxidant activity in vitro.
作者
徐福飞
庞佳慧
吴雨龙
江海涛
李盛杰
霍光明
华春
XU Fu-fei;PANG Jia-hui;WU Yu-long;JIANG Hai-tao;LI Sheng-jie;HUO Guang-ming;HUA Chun
出处
《饲料研究》
CAS
北大核心
2023年第9期71-79,共9页
Feed Research
基金
国家高技术研究发展计划(项目编号:2012AA021701)
国家自然科学基金项目(项目编号:21376112)
江苏省自然基金面上项目(项目编号:BK2014020929)
江苏省高校自然科学研究重大项目(项目编号:20KJA416003)
江苏省高校自然科学研究面上项目(项目编号:18KJD180002)
南京晓庄学院青年专项(项目编号:2019NXY47)。
关键词
超声波
液体双相渗透法
黄贞菌质
总黄酮
多糖
ultrasonic
liquid duplex penetration method
Huang Zhen mycoplasm
total flavonoids
polysaccharides
