摘要
从野生竹黄子实体分离获得能产生竹红菌素的无性株,经液态发酵培养及摇瓶正交试验获得最佳培养基配方和培养条件:蔗糖20g/L,酵母膏8g/L,玉米浆2g/L,pH6.0,摇瓶装量50/250ml(V/V),接种量10%(VV),培养温度28℃,摇床转速130r/min,培养周期96h,每升发酵液可获得色价为52的竹红菌素8g。本试验结果可为工业化生产竹红菌素提供参考。
A asexuae strain producing effective component hypocrellin was isolated from the hypha of Shiraia bambusicola sporocarp. By liquid-state fermentation and orthogonal teat with shake flask culture, the optimum medium component and culture conditions were： saccharose 20 g/L, yeast extract 8 g/L, maize extract 2 g/L, pH 6.0, culture broth quantity 50/250 mL（V/V） flask, inoculum 10% （V/V）, culture temperature 28℃, shake rotation 130 r/min, fermentation period 96 h. The culture can produce 8 g Hypocrellin（ E^1%11cm465 =52） per liter fermentation broth. This study will provide basis for the future massively industrialized hypcorllin production.
出处
《中药材》
CAS
CSCD
北大核心
2005年第12期1049-1051,共3页
Journal of Chinese Medicinal Materials
基金
国家技术创新基金资助项目(02CJ-13-01-16)
关键词
竹黄菌
无性株
竹红菌素
液态发酵
Shiraia bambusicola
Asexual strain
Hypocrellin
Liquid-state fermentation
