摘要
研究一株细菌红色素的结构和理化性质。采用95%乙醇溶液提取菌体色素,考察光照、温度、pH值、氧化剂、防腐剂、酸味剂、糖类、VC和金属离子对该色素稳定性的影响,将粗色素纯化后进行波谱结构研究。结果表明:色素最大吸收波长在533nm附近,结构鉴定为灵菌红素,其易溶于有机溶剂,在pH≤5时稳定性较好;色素对辐射及高质量浓度蔗糖的耐受力较强,对自然光、高温的耐受力较差;低质量浓度的苯甲酸钠、抗坏血酸和低体积分数的过氧化氢对色素稳定性影响不大;酸味剂和部分金属离子(Na+、K+、Mg2+、Ca2+、Al3+、Mn2+)对色素有增色作用,Fe3+对色素有明显的影响,Zn2+离子对色素基本无影响。
The structure and physico-chemical properties of red pigment prepared from cultured cells of a high-yield microbial strain conserved in our laboratory by extraction into 95% ethanol and sequential purification on macroporous resin column and silica gel column were investigated.The stability of the pigment to sunlight,temperature,pH value,hydrogen peroxide（H2O2） as an oxidant,sodium benzoate as a preservative,acidity agents,sucrose,vitamin C and metal ions was evaluated.Results showed that the red pigment was identified as prodigiosin by the methods of UV/vis,ESI-MS and 1H-NMR,with a maximum absorption peak at a wavelength of around 533 nm under acidic conditions.It was very easy to dissolve in organic solvents and had excellent stability at pH ≤ 5.In addition,the pigment exhibited strong tolerance to radiation and high sucrose concentration,but poor resistance to light and high temperature.Moreover,no significant effect of sodium benzoate,VC and H2O2 on the pigment was found at low concentrations.Acidity agents and the metal ions Na＋,Al3＋,Mn2＋,K＋,Mg2＋ and Ca2＋ exhibited hyperchromic effects on the pigments,while Fe3＋ had severe undesirable effect,and Zn2＋ had little effect.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第7期156-161,共6页
Food Science
基金
河西学院校长基金项目
关键词
细菌
红色素
结构
理化性质
bacteria
red pigment
structure
physicochemical properties
