摘要
探究野葛不溶性膳食纤维(LIDF)在不同碱体系中的提取工艺。以LIDF得率为指标,通过响应面法优化LIDF提取工艺,并分析其抗氧化活性。结果表明:最佳工艺分别为NaOH/CH_(3)OH体系,反应时间92 min、反应温度85℃、NaOH添加量3.5 g、CH_(3)OH添加量800 mL、超纯水添加量200 mL,在此条件下LIDF得率为62.77%;NaOH/CH_(4)N_(2)O体系,反应时间148 min、反应温度80℃、NaOH添加量7.5 g、CH_(4)N_(2)O添加量12 g、超纯水添加量900 mL,在此条件下LIDF得率为63.51%;NaOH/H_(2)O_(2)体系,反应时间150 min、反应温度70℃、NaOH添加量4.5 g、超声功率90 W、超纯水添加量200 mL,质量分数3%H_(2)O_(2)添加量800 mL,在此条件下LIDF得率为66.53%。NaOH/H_(2)O_(2)提取的LIDF对DPPH·有较好的清除率、NaOH/CH_(3)OH提取的LIDF对ABTS^(+)·有较好的清除率,LIDF有较好的还原力。
The extraction process of insoluble dietary fiber from Pueraria lobata(LIDF) in different alkali systems was studied. The extraction process of LIDF was optimized by response surface method, and the antioxidant activity of LIDF was analyzed. The results showed that the optimal processes were as follows: In NaOH/CH_(3)OH system, reaction time was 92 min, reaction temperature was 85 ℃, NaOH dosage was 3.5 g, CH_(3)OH dosage was 800 mL, and ultra-pure water dosage was 200 mL. Under these conditions, the LIDF yield was 62.77 %. In NaOH/CH_(4)N_(2)O system, reaction time was 148 min, reaction temperature was 80 ℃, NaOH dosage was 7.5 g, CH_(4)N_(2)O dosage was 12 g, and ultra-pure water dosage was 900 mL. Under these conditions, the LIDF yield was 63.51 %. In NaOH/H_(2)O_(2) system, reaction time was 150 min, reaction temperature was 70 ℃, NaOH dosage was 4.5 g, ultrasonic power was 90 W, ultra-pure water dosage was 200 mL, and mass fraction 3 % H_(2)O_(2)dosage was 800 mL. Under these conditions, the yield of LIDF was 66.53 %. The LIDF extracted from NaOH/H_(2)O_(2) had better clearance rate of DPPH·, the LIDF extracted from NaOH/CH_(3)OH had better clearance rate of ABTS^(+)·, and the LIDF had better reducing power.
作者
苏福联
王睛芳
廖璐婧
罗凯
黄东海
何美军
SU Fu-lian;WANG Jing-fang;LIAO Lu-jing;LUO Kai;HUANG Dong-hai;HE Mei-jun(Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Key Laboratory of Biology and Cultivation of Herb Medicine,Ministry of Agriculture and Rural Affairs,P.R.China,Enshi 445000,Hubei,China;School of Biology and Food Engineering,Hubei University for Nationalities,Enshi 445000,Hubei,China)
出处
《粮食与油脂》
北大核心
2023年第12期114-119,共6页
Cereals & Oils
基金
湖北省现代农业产业技术体系(HBHZD-ZB-2020-005)
现代农业产业技术体系建设专项(CARS-21)
湖北省农业科技创新中心2020年度重大科技研发项目(2020-620-000-002-04)
湖北省重点研发计划项目(2020BCA059)
恩施州科技计划项目(D20210006)。
关键词
野葛
不溶性膳食纤维
碱体系
抗氧化活性
Pueraria lobata
insoluble dietary fiber
alkali system
antioxidant activity
