摘要
目的 研究体适能与肠道菌群的关系并筛选具有提升体适能潜能的细菌分类。方法 招募44例成年健康男性参与研究,取得体适能数据,利用潜在剖面分析分为高/低体适能组;采集体能测试前人群粪便样本进行高通量16S r DNA测序;使用R对测序数据处理后分析菌群的α多样性、β多样性,明确不同体适能水平人群间肠道菌群差异;通过线性判别分析效应值方法筛选对差异贡献较大的细菌分类作为相应体适能水平相关分类群;最后分析各相关分类群之间及其与体适能指数的相关关系。结果 两组间α多样性未见显著差异,β多样性存在显著差异;毛螺菌科ND3007组和梭菌纲UCG-014目与高体适能水平相关;欧陆森氏菌属、粪便拟杆菌和凸腹真杆菌组与低体适能水平相关;高体适能相关细菌分类与低体适能相关细菌分类的丰度表现为负相关,低体适能群相关细菌分类与体适能评价指标的相关性较强。结论 肠道菌群组成与体适能水平相关,毛螺菌科ND3007组可被认为是具有潜在提升体适能能力的肠道细菌分类。
Objective To investigate the association of physical fitness levels with gut microbiota and find out the bacterial taxa with potential of improving physical fitness.Methods 44 healthy adult men were recruited for this study and the data on physical fitness was obtained.They were assigned to a high or low physical fitness group by using latent profile analysis.High⁃throughput sequencing was used to detect 16S rDNA collected from the fecal samples of volunteers before physical test.The alpha or beta diversity of intestinal flora was analyzed to deter⁃mine the differences in gut flora between the two groups.Linear discriminant analysis Effect Size was applied to screen the taxa contributing more to the diversity,which were considered as closely related to physical fitness level.Spearman correlation analysis was used to determine the correlation among the richness of taxa that contribute more to diversity and indexes related to physical fitness.Results There wahs no significant difference in the alpha diversity of gut flora between the two groups,whereas the beta diversity differed significantly.Lachnospiraceae ND3007 group and Clostridia UCG⁃014 were related to higher level of physical fitness,while Olsenella,Bacteroi⁃des stercoris and Eubacterium ventriosum group were associated with lower lever of physical fitness.The richness of taxa related to better physical fitness condition appeared to be negatively correlated with that of worse physical fitness condition,and the latter showed a stronger correlation with indexes related to physical fitness than the former.Conclusions The composition of microbiota planted in gastrointestinal tract was associated with physical fitness.Lachnospiraceae ND3007 group could be considered as latent taxa to help improve physical fitness condition.
作者
刘宇
傅亚婷
陈佳晖
张雄宝
常文军
LIU Yu;FU Yating;CHEN Jiahui;ZHANG Xiongbao;CHANG Wenjun(Department of Naval Environmental and Occupa-tional Health,Faculty of Naval Medicine,Naval Medical University of People′s Liberation Army,Shanghai 200000,China)
出处
《实用医学杂志》
CAS
北大核心
2023年第8期1002-1008,共7页
The Journal of Practical Medicine
关键词
体适能
胃肠道微生物组
生物信息学
高通量测序
16S
rDNA
physical fitness
gastrointestinal microbiome
computational biology
high⁃throughput nucleotide sequencing
16S rDNA
