摘要
炭疽病是辣椒生产上所面临最严重的病害之一,迫切需要可靠的方法在辣椒发育早期筛选抗病资源。炭疽病菌株CD的分生孢子采用两种接种方法,对辣椒‘ST-8’不同苗龄材料进行接种,通过对发病率、叶片卷曲率以及病斑大小的比较分析最佳的接种方法。结果表明,使用浓度为1×10^(6)个/mL的孢子悬浮液进行针刺点接法效果最佳,有望应用于大规模高通量辣椒抗炭疽病种质筛选。
Anthracnose is one of the most serious diseases in pepper production,there is an urgent need for reliable methods to screen for Colletotrichum disease resistance in the early pepper development.In this study,by using Colletotrichum strain CD,we have introduced two methods to inoculate pepper‘ST-8’seedling:infiltration and needle point,and tried to find the optimal way by comparing the plant infection rate,leave curl rate and the size of necrotic disease spots.The results showed that the results obtained by using the needle point method under the resuspend spore concentration of 1×10^(6) cells/mL were the best,which is expected to be applied to large-scale screening of pepper being resistant to anthracnose.
作者
李月
余婉贤
李宁
姚明华
李峰
邓颖天
LI Yue;YU Wan-xian;LI Ning;YAO Ming-hua;LI Feng;DENG Ying-tian(Key Laboratory of Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,Wuhan 430070;Institute of Cash Crops,Academy of Agricultural Science Hubei Province,Wuhan 430064)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第4期221-226,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(31972420,32172600)
国家重点研发计划(2019YFD1000301)。
关键词
辣椒
苗期
炭疽菌
接种方法
Capsicum annuum L.
seedling
Colletotrichum
inoculation method
