摘要
为明确香花油茶(Camellia osmantha)不同无性系苗期炭疽病发生情况及其病菌致病力分化状况,为香花油茶苗期炭疽病的综合防治和抗病育种提供理论依据,采用平行线取样法,对27个香花油茶无性系苗期炭疽病进行调查与统计,通过形态特征和分子系统学分析对炭疽病病菌进行鉴定,并采用离体叶片接种法对所获菌株进行致病性及致病力测定,明确各菌株的致病力等级。结果表明,2019—2021年,不同无性系炭疽病发病率及病情指数均呈上升趋势,均在2019—2020年增长较快;27个菌株的培养性状及形态特征均相似,鉴定为Colletotrichum fructicola;来自6和12号无性系的炭疽病菌株为强致病力菌株,其他25个无性系的炭疽病菌株均为中等致病力菌株。香花油茶无性系苗期炭疽病菌C.fructicola致病力分化明显,以中等致病力菌株为优势群体。
In order to confirm occurrence of anthracnose of different Camellia osmantha clones at seedling stage and differentiation status of pathogenicity of bacteria,and provide theoretical basis for comprehensive control of anthracnose of C.osmantha at seedling stage and breeding of improved varieties for disease resistance,anthracnose in 27 C.osmantha clones at seedling stage were investigated and analyzed by parallel lines,anthracnose bacteria were identified by morphological characteristics and molecular phylogenetic analysis,virulence and pathogenicity of strains were determined by inoculation method of isolated leaves,and pathogenicity grade of strains was clarified.Results showed that disease incidences and disease indexes of anthracnose in different clones increased gradually from 2019 to 2021,especially from 2019 to 2020.Culture characters and morphological characteristics of 27 strains were similar,which were identified as Colletotrichum fruticola.Pathogenicity of strains from clones 6 and 12 were strong,and pathogenicity of strains from other 25 clones were medium.Pathogenicity of C.fruticola in C.osmantha clones at seedling stage were significantly different,and medium pathogenic strains were dominant group.
作者
廖旺姣
钟雅婷
韦维
邹东霞
罗辑
Liao Wangjiao;Zhong Yating;Wei Wei;Zou Dongxia;Luo Ji(Guangxi Forestry Research Institute,Guangxi Key Laboratory of Special Non-wood Forest Cultivation&Utilization,Guangxi Engineering Research Center of Natural Enemy Breeding of Forestry Pests,Nanning,Guangxi 530002,China)
出处
《广西林业科学》
2022年第5期676-685,共10页
Guangxi Forestry Science
基金
广西特色经济林培育与利用重点实验室自主课题(19-A-02-01)。
关键词
香花油茶
炭疽病
致病力分化
无性系苗木
Camellia osmantha
anthocnose
pathogenicity differentiation
clonal seedling
