摘要
以“贵长”猕猴桃病果为试材,通过病原菌分离、形态学鉴定、分子生物学鉴定及致病力测定,研究了“贵长”猕猴桃蒂腐病病原菌多样性及致病力差异,以期为猕猴桃蒂腐病防控提供参考依据。结果表明:“贵长”猕猴桃蒂腐病病原菌有4类,8株,分别是小新壳梭胞菌属(Neofusicoccum sp.)、间座壳菌属(Diaporthe sp.)、葡萄座腔菌(Botryosphaeria sp.)、盘多毛胞菌属(Pestalotiopsis sp.),各菌属检出率为25.0%、50.0%、12.5%、12.5%;将菌株序列上传至GenBank,获得登录号MK5875050-MK587753、MK587756、MK587760、MK587762、MK5875063;聚类分析将8株病原菌致病力分为强致病力(37.5%)、中致病力(25.0%)和弱致病力(37.5%),各菌株致病力差异与其所属菌属无相关性。
Taking‘Guichang’kiwifruit as the test material,the stem-end rot pathogens′diversity and pathogenicity of‘Guichang’kiwifruit were studied by pathogen isolation,morphological identification,molecular biological identification and pathogenicity determination,in order to provide reference for the prevention of the kiwifruit stem-end rot.The results showed that there were 8 pathogen strains belonging to 4 categories,respectively are Neofusicoccum sp.,Diaporthe sp.,Botryosphaeria sp.and Pestalotiopsis sp.,and the detection rates were 25.0%,50.0%,12.5%,12.5%.The strain sequence was uploaded to GeneBank and login numbers were MK5875050-MK587753,MK587756,MK587760,MK587762,MK5875063.The pathogenicity of 8 isolates was classified into strong pathogenicity(37.5%),moderate pathogenicity(25.0%)and weak pathogenicity(37.5%)by cluster analysis.There was no correlation between the pathogenicity of each strain and its genus.
作者
雷霁卿
曾傲敏
刘颖
曹森
王瑞
LEI Jiqing;ZENG Aomin;LIU Ying;CAO Sen;WANG Rui(Food and Pharmaceutical Engineering College,Guiyang University,Guiyang,Guizhou 550005)
出处
《北方园艺》
CAS
北大核心
2022年第21期31-37,共7页
Northern Horticulture
基金
贵阳市科技计划资助项目(筑科合同[2019]11-7)
贵州省大学生创新创业训练计划资助项目(202110976057)
贵阳市财政支持贵阳学院学科建设与研究生教育资助项目(2021-xk14)
关键词
猕猴桃
蒂腐病
病原菌
kiwifruit
stem-end rot
pathogenic bacteria
