摘要
目的探讨长链非编码RNA(LncRNA)FGD5-AS1、LncRNA-FAS-AS1在慢性牙周炎患者龈沟液中的表达及其与病原菌感染、病情严重程度的相关性。方法选取2020年1月-2022年6月本院收治的168例慢性牙周炎患者为研究对象(观察组),根据患者病情程度分为轻度组(74例)、中度组(51例)和重度组(43例),同期选取健康体检者150例为对照组。收集一般资料,比较龈沟液lncRNA FAS-AS1、lncRNA FGD5-AS1水平;采用Pearson相关分析龈沟液lncRNA FAS-AS1、lncRNA FGD5-AS1表达与牙周指标及病原菌感染的相关性;利用受试者工作特征(ROC)曲线评估lncRNA FAS-AS1、lncRNA FGD5-AS1水平对慢性牙周炎患者的诊断价值。结果观察组患者龈沟液lncRNA FAS-AS1(0.64±0.15)、lncRNA FGD5-AS1(0.52±0.14)表达水平均低于对照组[(1.01±0.18)、(1.09±0.25)],PD、AL、PLI、SBI水平均高于对照组,差异有统计学意义(P<0.05);龈沟液lncRNA FAS-AS1、lncRNA FGD5-AS1水平随病情程度的加重而降低,其中重度组慢性牙周炎患者龈沟液lncRNA FAS-AS1(0.41±0.10)、lncRNA FGD5-AS1(0.29±0.12)表达水平低于轻度组[(0.79±0.17)、(0.67±0.15)]、中度组[(0.62±0.15)、(0.51±0.14)],中度组患者龈沟液lncRNA FAS-AS1、lncRNA FGD5-AS1表达水平低于轻度组,差异均有统计学意义(P<0.05);观察组共检出病原株249株,对照组共检出37株。观察组牙龈卟林单胞菌、伴放线放线菌、福赛类杆菌检出率均高于对照组,差异均有统计学意义(P<0.05);Pearson相关性分析显示,龈沟液lncRNA FAS-AS1、lncRNA FGD5-AS1表达水平与慢性牙周炎患者发生病原菌感染的点二列相关系数分别为-0.782、-0.713,差异有统计学意义(P<0.05);慢性牙周炎患者龈沟液中lncRNA FGD5-AS1、lncRNA FAS-AS1表达与PD、AL、PLI、SBI均呈负相关(P<0.05);ROC曲线显示,龈沟液lncRNA FAS-AS1、lncRNA FGD5-AS1水平诊断慢性牙周炎患者的曲线下面积(AUC)分别为0.914、0.890,联合诊断的AUC为0.950,较单一指标检测�
Objective To investigate the expression of long non-coding RNA(LncRNA)FGD5-AS1 and LncRNA-FAS-AS1 in gingival crevicular fluid of patients with chronic periodontitis and their correlation with pathogenic bacteria infection and severity of disease.Methods A total of 168 patients with chronic periodontitis admitted to our hospital from January 2020 to June 2022 were regarded as the study subjects(observation group),the patients were grouped into mild group(74 cases),moderate group(51 cases)and severe group(43 cases)according to the severity of disease,meantime,150 healthy people were regarded as the control group.General data were collected,and the levels of lncRNA FAS-AS1 and lncRNA FGD5-AS1 in gingival crevicular fluid were compared;Pearson correlation was applied to analyze the correlation between the expression of lncRNA FAS-AS1 and lncRNA FGD5-AS1 in gingival crevicular fluid and periodontal index and pathogenic bacteria infection;the diagnostic value of the levels of lncRNA FAS-AS1 and lncRNA FGD5-AS1 in patients with chronic periodontitis was evaluated by the receiver operating characteristic(ROC)curve.Results The expression levels of lncRNA FAS-AS1(0.64±0.15)and lncRNA FGD5-AS1(0.52±0.14)in gingival crevicular fluid of patients in the observation group were lower than those in the control group[(1.01±0.18),(1.09±0.25)],and the levels of PD,AL,PLI and SBI were obviously higher than those in the control group(P0.05);the levels of lncRNA FAS-AS1 and lncRNA FGD5-AS1 in gingival crevicular fluid decreased with the aggravation of the disease,the expression levels of lncRNA FAS-AS1(0.41±0.10)and lncRNA FGD5-AS1(0.29±0.12)in gingival crevicular fluid of patients with chronic periodontitis in severe group were lower than those in mild group[(0.79±0.17),(0.67±0.15)]and moderate group[(0.62±0.15),(0.51±0.14)],the expression levels of lncRNA FAS-AS1 and lncRNA FGD5-AS1 in gingival crevicular fluid of patients in the moderate group were lower than those in the mild group(P0.05);249 pathogenic strains were dete
作者
刘从厚
江凤川
葛大量
LIU Cong-hou;JIANG Feng-chuan;GE Da-liang(General Dental Department of Jianyang People's Hospital,Sichuan 641400,China;Department of Stomatology,Dazhu County People's Hospital)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第4期451-455,共5页
Journal of Pathogen Biology
