摘要
目的探究汉黄芩素对鼻咽癌细胞C666-1、HK-1增殖、迁移和侵袭能力的影响。方法采用CCK8实验明确汉黄芩素对鼻咽癌细胞C666-1、HK-1的活性抑制作用,并计算IC_(50);采用平板集落形成实验明确汉黄芩素对鼻咽癌细胞C666-1增殖和独立生存能力的影响;采用划痕实验和Transwell迁移实验研究汉黄芩素对鼻咽癌细胞迁移能力的影响;最后采用Transwell侵袭实验研究汉黄芩素对鼻咽癌细胞侵袭能力的影响。结果CCK8实验结果显示,汉黄芩素对C666-1和HK-1细胞的活力均表现出明显的抑制作用,随药物浓度增加,抑制效果明显增强;同一药物浓度下,药物作用48 h的抑制效果高于24 h,抑制效果表现为剂量时间依赖性;汉黄芩素作用于C666-1细胞24 h的IC_(50)值为37.15μmol/L、48 h为20.75μmol/L;汉黄芩素作用于HK-1细胞24 h的IC_(50)值为29.09μmol/L、48 h为20.46μmol/L。平板集落实验结果显示,汉黄芩素干预后,鼻咽癌细胞C666-1细胞形成肉眼可见集落的数目更少,体积更小。划痕实验和Transwell迁移实验显示,C666-1及HK-1细胞的迁移能力明显受到抑制,且药物浓度越高,抑制作用越强。Transwell侵袭实验显示,汉黄芩素干预后,穿过基质胶后迁移至下室的细胞数目明显减少。结论汉黄芩素能抑制鼻咽癌细胞的活性、增殖、迁移以及侵袭的能力。
Objective To investigate the effects of wogonin on the proliferation,migration and invasion of nasopharyngeal carcinoma cells C666-1 and HK-1.Methods CCK8 assay was used to determine the inhibitory effect of wogonin on the activity of nasopharyngeal carcinoma cells C666-1 and HK-1,and IC_(50)was calculated.The effect of wogonin on the proliferation and independent viability of nasopharyngeal carcinoma cell C666-1 was determined by plate colony formation assay.The effect of wogonin on the migration ability of nasopharyngeal carcinoma cells was studied by scratch test and Transwell migration test.Finally,Transwell invasion assay was used to study the effect of wogonin on the invasion ability of nasopharyngeal carcinoma cells.Results The results of CCK8 assay showed that wogonin significantly inhibited the viability of C666-1 and HK-1 cells,and the inhibitory effect was significantly enhanced with the increase of drug concentration.At the same drug concentration,the inhibitory effect of 48 h was higher than that of 24 h,and the inhibitory effect was dose-time dependent.The IC_(50)of wogonin on C666-1 cells was 37.15μmol/L at 24 h and 20.75μmol/L at 48 h.The IC_(50)value of wogonin on HK-1 cells was 29.09μmol/L at 24 h and 20.46μmol/L at 48 h.The results of plate colony assay showed that after wogonin intervention,the number of visible colonies formed by nasopharyngeal carcinoma cells C666-1 cells was less and the volume was smaller.Scratch test and Transwell migration assay showed that the migration ability of C666-1 and HK-1 cells was significantly inhibited,and the higher the drug concentration,the stronger the inhibition.Transwell invasion assay showed that after wogonin intervention,the number of cells migrating to the lower chamber after passing through matrigel was significantly reduced.Conclusion Wogonin can inhibit the activity,proliferation,migration and invasion of nasopharyngeal carcinoma cells.
作者
林彩星
陈罡
杨振东
蒋伟哲
言柯柯
伍小薇
韦祝新
LIN Cai-xing;CHEN Gang;YANG Zhen-dong;JIANG Wei-zhe;YAN Ke-ke;WU Xiao-wei;WEI Zhu-xin(Department of Radiation Oncology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Pathology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;School of Pharmacy,Guangxi Medical University,Nanning 530021,Guangxi,China)
出处
《医学信息》
2023年第10期71-76,共6页
Journal of Medical Information
关键词
鼻咽癌
汉黄芩素
迁移
侵袭
Nasopharyngeal carcinoma
Wogonin
Migration
Invasion
