摘要
目的:探讨阿司匹林诱导同源不同辐射抗拒能力鼻咽癌细胞株CNE2R/CNE2凋亡的作用及其机制。方法:采用MTT法、流式细胞术和Western blot法检测并比较阿司匹林对CNE2R和CNE2细胞活力、细胞凋亡及凋亡相关蛋白procaspase-3、cleaved caspase-3、procaspase-9、procaspase-12、PARP、cleaved PARP、Bcl-2和Bax,以及PI3K p110α、Akt和p27蛋白水平的影响。结果:阿司匹林抑制同源不同辐射抗拒能力细胞CNE2R/CNE2的活力(阿司匹林对CNE2细胞作用24、48和72 h的IC50分别为6.18、3.92和3.06 mmol/L,对CNE2R细胞作用24、48和72 h的IC50分别为7.05、3.90和2.20 mmol/L,两者差异无统计学显著性)。阿司匹林作用48 h后,CNE2R细胞凋亡率升高,明显高于CNE2细胞(P<0.05)。阿司匹林作用48 h后,CNE2和CNE2R细胞中procaspase-3、procaspase-9、procaspase-12和PARP的蛋白水平降低,cleaved caspase-3和cleaved PARP的蛋白水平升高(P<0.05);PI3K p110α和Akt蛋白水平下降,Bcl-2水平降低,Bax水平升高,Bcl-2/Bax比值降低,p27水平升高(P<0.05)。结论:阿司匹林对同源不同辐射抗拒能力鼻咽癌细胞株CNE2R和CNE2具有同等细胞活力抑制作用;并且可以诱导细胞凋亡,且对抗辐射细胞株CNE2R的凋亡诱导作用更明显。阿司匹林的抗癌作用可能与其影响PI3K/Akt信号通路及其下游蛋白的水平有关。
AIM：To investigate the effects of aspirin on the apoptosis of nasopharyngeal carcinoma cell lines CNE2 R and CNE2 with different radioresistance and its potential mechanism.METHODS：The effects of aspirin on the cell viability,apoptosis,and protein levels of procaspase-3,cleaved caspase-3,procaspase-9,procaspase-12,PARP and cleaved PARP,PI3 K p110α,Akt,Bcl-2,Bax and p27 in the CNE2 R cells and CNE2 cells were detected by the methods of MTT assay,flow cytometry and Western blot.RESULTS：Aspirin inhibited the viability of homologous nasopharyngeal carcinoma cell lines CNE2 and CNE2 R（with the IC50 to CNE2 cells of 6.18,3.92 and 3.06 mmol/L for 24 h,48 h and72 h,respectively;and with the IC50 to CNE2 R cells of 7.05,3.90 and 2.20 mmol/L for 24 h,48 h and 72 h,respectively）.After treated with aspirin for 48 h,the apoptotic rate of CNE2 R cells was higher than that of CNE2 cells（P 〈0.05）.After treated with aspirin for 48 h,the protein levels of procaspase-3,procaspase-9,procaspase-12 and PARP were decreased,the protein levels of cleaved caspase-3,cleaved PARP and p27 were increased,and the protein levels of PI3 K p110α,Akt and Bcl-2/Bax were decreased.CONCLUSION：Aspirin inhibits the viability of homologous nasopharyngeal carcinoma cell lines CNE2 R and CNE2 with different radioresistance.Aspirin also induces the apoptosis of CNE2 and CNE2 R cells,which is more effective in CNE2 R cells.The underlying mechanisms may be involved in affecting PI3 K/Akt signaling pathway,Bcl-2/Bax and p27 expression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2017年第11期1980-1986,共7页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81372410)
广东省科技计划(No.2013B031800002
No.2015A020211006)
关键词
鼻咽癌
辐射抗拒性
阿司匹林
细胞凋亡
Nasopharyngeal neoplasms
Radioresistance
Aspirin
Apoptosis
