摘要
本文建立高效液相色谱法-荧光检测器测定蜂蜜中的氧氟沙星药物残留量的方法。提取分离时蜂蜜和NaOH溶液的比例为1:1,蜂蜜和乙酸乙腈溶液比例为1:5。色谱条件:0.2%甲酸溶液—乙腈为流动相,比例为87:13,35℃柱温,1.5 mL·min-1流速,荧光检测280 nm激发波长,发射波长450 nm。实验结果表明,此方法简单,成本低,效率高,实用性强。
This study established a method for the determination of ofloxacin residues in honey by high-performance liquid chromatography with fluorescence detector.It was showed the ration of honey to sodium hydroxide solution was 1:1,and the ration of honey to acetonitrile acetate solution was 1:5.The chromatographic conditions were as follows:0.2%formic acid solution-acetonitrile as the mobile phase,with a ratio of 87:13,a column temperature of 35℃,a flow rate of 1.5 mL·min~(-1),a fluorescence detection excitation wavelength of 280 nm,and an emission wavelength of 450 nm.The experimental results show that this method is simple,low cost,high efficiency,and practical.
作者
高倩妮
魏超田
陆宁
姚梦靖
周晓龙
GAO Qianni;WEI Chaotian;LU Ning;YAO Mengjing;ZHOU Xiaolong(Biology and Food Engineering Department,Bozhou University,Bozhou 236800,China;Bozhou Key Laboratory of Functional Food with Medicine food homology,Bozhou 236800,China)
出处
《现代食品》
2023年第4期186-188,共3页
Modern Food
基金
安徽高校自然科学研究项目(2022AH052411)
教学质量与教学改革工程项目(2021XJXM048)
科研平台专项研究项目(BYP2021Z05)
安徽省大学生创新创业项目(2021XJXM130)。
关键词
高效液相色谱
蜂蜜
氧氟沙星
high performance liquid chromatography
honey
ofl oxacin
