摘要
建立了高效液相色谱法测定鸡肉中甲氧苄氨嘧啶残留量的方法.试样中残留的甲氧苄氨嘧啶用酸性甲醇提取,提取液调至碱性后再用二氯甲烷液液分配,浓缩后,残渣用甲醇-0.5%高氯酸水溶液(30+70)溶解,经三氯甲烷净化后,用Inertsil ODS-3(250mm × 4.6mm(id),5μm)柱分离,以甲醇-0.5%高氯酸(30+70)作流动相,流速1.0mL·min-1,于230nm波长处检测.结果表明,样品的加标平均回收率为94.6%～81.2%,相对标准偏差为2.88%～4.31%,方法的测定低限(LOQ)为0.04mg·kg-1.该方法具有简便、快捷、灵敏、重现性好.
A high performance liquid chromatographic method （HPLC） was developed for the determination of trimethoprim residue in chicken. The trimethoprim residue in the sample was extracted with a solution of acidic methanol. The supermatant liquid after is regulated to alkaline is partitioned with dichloromethane. The dichloromethane solution is evaporated and residues is dissolved with methanol - 0.5 % perchloric acid aqueous solution （ 3 ＋ 7）. The solution is then cleaned up with chloroform. The chromatographic separation was performed on a Inertsil ODS- 3 column （ 250mm × 4.6mm （ id ）, 5μn ） using methanol - 0,5 % perchloric acid aqueous solution（ 30：70 ）, v/v） as moble phase at a flow rate of 1,0mL· min^-1 and UV detector at 230nm, The results showed that the average recoveries were 93,2% 81.2 % and the relative standard deviations were 2.88 % - 4.31%, The limit of determination of this method is 0.04mg · kg^- 1, The method is simple, rapid, sensitive and reproducible.
出处
《化学工程师》
CAS
2005年第12期19-21,共3页
Chemical Engineer
