摘要
目的:探讨榆干离褶伞溶栓酶(LUFE)对高脂血症大鼠肝损伤的保护作用及其机制。方法:以高脂饮食建立高脂血症模型,用不同剂量的LUFE灌胃模型大鼠。HE染色观察肝脏病理形态学变化;比色法检测大鼠血浆生化指标;蛋白免疫印迹法观察炎症相关蛋白的表达水平。结果:LUFE可以缓解高脂血症大鼠的肝组织损伤,降低血浆血脂水平、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、巨噬细胞趋化蛋白-1(MCP-1)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平,下调TLR4、MyD88和磷酸化的PI3K、Akt、NF-кB水平。结论:LUFE通过调控PI3K/Akt/NF-кB和TLR4/MyD88/NF-кB信号通路,可以保护高脂血症所致的大鼠肝损伤。
Objective:To investigate the protective effect and mechanism of Lyophyllum ulmarium fibrinolytic enzyme(LUFE)on rats with hyperlipidemia.Methods:Hyperlipidemia models were established by feeding with a high-fat diet,and the model rats were gavaged with different doses of LUFE.HE stain was used to analyze the morphological changes of liver.The biochemical indexes of rat plasma were detected by colorimetric method.WB analyses the expression level of related proteins.Results:LUFE could alleviate the liver tissue injury of hyperlipidemia rats and reduce the plasma lipid level and IL-6,TNF-α,MCP-1,ALT,AST.LUFE can down regulate TLR4,MyD88,phosphorylated PI3K,phosphorylated Akt and phosphorylated NF-кB protein level.Conclusion:LUFE protects against hyperlipidemia-induced liver injury in rats by regulating PI3K/Akt/NF-кB and TLR4/MyD88/NF-кB signaling pathways.
作者
李钰
苏新
张俊慧
张蕊萌
沈明花
LI Yu;SU Xin;ZHANG Jun-hui;ZHANG Rui-meng;SHEN Ming-hua(Medical College of Yanbian University,Yanji,Jilin 133000,China)
出处
《食品与机械》
CSCD
北大核心
2023年第4期156-162,共7页
Food and Machinery
基金
国家自然科学基金地区科学基金项目(编号:81760031)
吉林省自然科学基金项目(编号:YDZJ202201ZYTS212)。
关键词
榆干离褶伞
溶栓酶
高脂血症
肝损伤
炎症
Lyophyllum ulmarium
fibrinolytic enzyme
hyperlipidemia
liver injury
inflammation
