摘要
刺参内脏作为海参初加工的主要副产物,具有良好的营养和开发利用价值。文章研究了利用酶解法从刺参内脏中制备ACE抑制肽的工艺。通过单因素实验和响应面优化法确定了胰蛋白酶酶解制备海参内脏ACE抑制肽的最佳条件为:温度37℃、pH8.5、料液比1:1.98、加酶量2.56%、酶解时间1.0 h,所得内脏多肽ACE抑制率为56.80%。通过超滤分级、Sephadex G-25分子筛层析柱层析,对活性肽进一步纯化,得到最优组分ACE抑制率为50%时抑制浓度0.535 mg/mL,通过高效液相色谱-串联质谱(HPLC-MS/MS)分析鉴定,得到多肽氨基酸序列组成和分子质量等信息。该研究为刺参内脏综合利用和食源性ACE的开发提供了基础。
The viscera of sea cucumber,as the main by-product of the primary processing of sea cucumber,has good nutritional and development value.In this study,the preparation of ACE inhibitory peptide from the viscera of sea cucumber by enzymolysis was studied.Through single factor experiment and response surface optimization method,the optimal conditions of trypsin enzymatic hydrolysis to prepare the visceral ACE inhibitory peptide of sea cucumber were determined as follows:temperature 37℃,pH 8.5,material to liquid ratio 1:1.98,enzyme amount 2.56%,and enzymatic hydrolysis time 1.0 h.The ACE inhibitory rate of visceral polypeptide was 56.80%.The active peptide was further purified by ultrafiltration fractionation and Sephadex G-25 molecular sieve chromatographic column chromatography,and the inhibitory concentration was 0.535 mg/mL when the ACE inhibitory rate of the optimal component was 50%.It was identified by HPLC MS/MS analysis,and the amino acid sequence composition and molecular weight of the polypeptide.This study provides a basis for the comprehensive utilization of the internal organs of sea cucumber and the development of food derived ACE.
作者
杨林彤
姜雨辰
周建华
焦绪栋
YANG Lintong;JIANG Yuchen;ZHOU Jianhua;JIAO Xudong(School of Life Sciences,Yantai University,Yantai 264005,China;Yantai Coastal Zone Research Institute,Chinese Academy of Sciences,Yantai 264003,China;Shandong Yuanlide Marine Biomedicine Technology Limited Company,Penglai 265600,China;Shandong Shenliwei Biotechnology Limited Company,Weihai 264200,China)
出处
《食品科技》
CAS
北大核心
2023年第3期162-169,共8页
Food Science and Technology
基金
烟台市科技计划项目(2021XDHZ059)。
关键词
刺参内脏
ACE抑制肽
酶解制备
响应面优化
分离纯化
sea cucumber viscera
ACE inhibitory peptide
enzymatic hydrolysis
response surface optimization
separation and purification
