摘要
研究杉木(Cunninghamia lanceolata)生长过程中土壤养分、土壤细菌群落变化特征,有助于了解杉木经营中地力衰退的成因。采用Illumina PE250平台测定了幼林(3年)、中龄林(8年)、近熟林(12年)、成熟林(21年)的土壤细菌群落多样性,并研究了其与土壤养分等环境因子的相关性。结果表明:(1)随着林龄增长,除有效磷(AP)外,土壤有机碳(SOC)及有效氮(AN)、有效钾(AK)并未出现下降趋势,中龄林的SOC、AN、AP、AK相对最高。(2)细菌群落中酸杆菌门(43.79%—57.66%)、变形菌门(14.21%—31.83%)、绿弯菌门(11.52%—24.23%)的菌群占优势地位。(3)成熟林土壤细菌群落中OTU数量最少,Chao指数也最低。对于细菌群落Shannon-Wiener指数,A层土壤(0—20 cm)为幼林最高,成熟林最小,B层土壤(20—40 cm)为中龄林最高,近熟林最低。(4)基于Unweighted Unifrac距离的RDA分析表明,杉木人工林细菌群落组成主要受坡度、海拔、树高、AK的影响,其中坡度、树高、海拔更多解释林龄间的差异,而AK更多解释土壤层次间的差异。研究表明,研究区杉木林土壤养分并未随着林龄增长而下降;林龄间细菌群落组成差异主要受坡度、树高、海拔影响,AK主要引起土壤层次间细菌群落的差异。
The study on the change characteristics of soil nutrient and soil bacterial community during the growth of Cunninghamia lanceolata is helpful to understand the causes of soil fertility decline in the management.The soil bacterial community diversity of young forest(3 years),half-mature forest(8 years),near-mature forest(12 years),mature forest(21 years)was measured using Illumina PE250 platform,and its correlations with soil nutrients and other environmental factors were also studied.The results are as follows.(1)With the increase of stand age,soil organic carbon(SOC)and available nitrogen(AN)and available potassium(AK)showed no decreasing trend except available phosphorus(AP).The SOC,AN AP and AK of mature forest were the highest.(2)In the bacterial community,Acidobacteria(43.79%-57.66%),Proteobacteria(14.21%-31.83%),and Camisoliaceae(11.52%-24.23%)account for a high proportion.(3)The numbers of OTU and Chao index of soil bacterial community in mature forest were the lowest.For Shannon-Wiener index of bacterial community,the highest value was found in young forest and the lowest value in mature forest in layer A soil(0-20 cm),the highest value was found in half-mature forest and the lowest value was found in near-mature forest in layer B soil(20-40 cm).(4)RDA analysis based on the distance of Unweighted Unifrac showed that the bacterial community composition of Cunninghamia lanceolata plantation was mainly affected by slope,altitude,tree height and AK,among which,slope,tree height and altitude explained the difference between stand ages more,while AK explained the difference between soil layers more.The results showed that soil nutrients did not decrease with the increase of stand age.The difference of bacterial community composition between stand ages was mainly affected by slope,tree height and altitude,and AK mainly caused the difference of bacterial community between soil layers.
作者
郑威
彭玉华
申文辉
谭长强
董利军
雷震
何峰
陈始贵
ZHENG Wei;PENG Yuhua;SHEN Wenhui;TAN Zhangqiang;DONG Lijun;LEI Zhen;HE Feng;CHEN Shigui(Guangxi Forestry Research Institute,Nanning 530002,China;Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning 530002,China;Key Laboratory of Central South Fast-growing Timber Cultivation of National Forestry and Grassland Administration,Nanning 530002,China;Beijiang River State Forest Farm of Rongshui Miao Autonomous County,Rongshui 545300,China)
出处
《生态科学》
CSCD
2023年第3期37-45,共9页
Ecological Science
基金
广西科技重大专项课题(桂科AA17204087-8)
广西优良用材林资源培育重点实验室自主课题(17-A-02-01)。
关键词
杉木人工林
细菌群落
多样性
高通量测序
Cunninghamia lanceolata plantation
bacterial community
diversity
high-throughput sequencing
