摘要
为探明杉木人工林土壤真菌遗传多样性及其与环境因子的关系,采用454测序技术对土壤真菌的遗传多样性进行了分析,测定了黄丰桥林场杉木人工林土壤真菌的遗传多样性与环境因子的相关性。试验结果表明:①不同代数、林龄的杉木人工林土壤理化性质及林下植被多样性均有显著差异。第1代杉木幼林林土壤肥力较高,有机质、全N、速效K的均值分别为88.02g/kg、2.56 g/kg、84.96 mg/kg均高于第2代和第3代杉木幼林林,速效N和含水量的均值分别为22.86 mg/kg和26.28%低于其他样地。杉木幼林林下植被多样性最为丰富。②通过454测序技术分析发现第1代杉木幼林真菌Ace丰富度指数、Chao丰富度指数及群落遗传多样性指数均大于第2代杉木幼林和第3代杉木幼林。杉木人工林土壤中粪壳菌纲(Sordariomycetes)真菌为优势种群。不同栽培代数杉木人工林的真菌群落存在差异,其中块菌科(Tuberaceae)为第2代和第3代杉木林特有真菌,而不同发育阶段的杉木人工林的真菌群落差异不明显。③经RDA分析,杉木人工林土壤主要真菌群落受含水量、有机质、速效P、速效K影响较大。土壤真菌群落遗传多样性Shannon-Wiener多样性指数与林下植被多样性、土壤全N显著正相关,土壤真菌Chao指数与土壤真菌Shannon-Wiener多样性指数、土壤全N含量显著正相关。本研究表明不同栽培代数杉木人工林的真菌群落存在差异,土壤真菌群落与环境因子之间具有相关性。
Cunninghamia lanceolata（ Lamb. ）Hook. （Chinese fir） is one of the most important industrial timber species in China, the area of Chinese fir plantation accounts for 21.35% of the total plantation area in China. At present, the Chinese fir plantation has some problems such as continuous planting, single tree species, mismatch tree species with site and so on, this contributed to productivity decline and soil degradation. Remarkable changes caused by soil degradation, for instance, soil microorganism, soil physic-chemistry characteristics, soil enzyme activity and undergrowth vegetation, etc. Soils represent a huge reservoir of biodiversity with several billion prokaryotic and eukaryotic microorganisms, corresponding to numerous different taxa, inhabiting a single gram of soil. Fungi are the dominant eukaryotic lineage in terms of biomass in soil, where they play key roles as decomposers, pathogens, and mycorrhizal mutualists. Due to their large number of species, specialization, and important ecological functions, fungi are also excellent bioindicators. Investigating the fungal diversity becomes crucial for the ecological characterization of any given site. Traditionally, diversity was assessed using selective plating and direct viable counts. Limitations include uncultivable microorganisms not detected, bias towards fast growing individuals, and bias towards fungal species produce largequantities of spores. allows identification plate count. High -throughput of fungal as well tag-encoded FLX amplicon pyrosequencing has been used to as the prediction of phylogenetic relationships. This method This study aims to provide an overview of soil fungal diversity in soils of Chinese fir relationship between soil fungal diversity and environmental factors. To achieve the objectives, study fungal diversity and avoided the limitations of plantations and examine the we employed high-throughput tag-encoded FLX amplicon pyrosequencing to analyze fifteen soil samples from the Chinese fir plantation forests at the Huangf
出处
《生态学报》
CAS
CSCD
北大核心
2014年第10期2725-2736,共12页
Acta Ecologica Sinica
基金
国家林业局重点项目(2012-01)
中南林业科技大学研究生科技创新基金资助项目(2010bx02)
关键词
杉木人工林
真菌遗传多样性
真菌群落
454测序
Chinese Fir Plantation
fungal genetic diversity
fungal community
454 pyrosequencing
