摘要
【目的】研究冀北山区不同植被恢复类型对根际土壤细菌群落结构特征和多样性影响,探索自然和人工恢复对土壤微生物的作用机制,为该区域植被恢复与管理提供理论依据。【方法】以自然恢复的灌草丛、灌木林、次生林以及人工恢复的人工林根面、根际和非根际土为研究对象,通过Illumina Miseq平台对细菌16S rRNA的V3—V4片段进行高通量测序,分析土壤细菌α、β和功能多样性、群落结构组成以及与环境因子的关系。【结果】1)各恢复类型细菌α多样性差异显著(P<0.05),次生林土壤细菌丰度和多样性均高于人工林,灌草丛多样性高,丰度最低;灌木林丰度高,多样性最低。不同根际范围土壤细菌α多样性差异不显著(P>0.05),整体表现为根面和根际土高于非根际土。2)次生林和人工林土壤细菌群落结构有一定相似性;次生林与灌草丛、灌木林土壤细菌群落结构差异较大。根际土与非根际土细菌群落结构有一定相似性,根面土与根际、非根际土细菌结构差异较大。3)变形菌门、放线菌门、拟杆菌门、酸杆菌门、芽单胞菌门和厚壁菌门为优势类群。各样地有机质分解菌群(变形菌门、放线菌门和拟杆菌门)丰度差异显著,灌草丛和次生林显著高于其他样地,根面和根际土显著高于非根际土。固碳和固氮菌群(芽单胞菌门和厚壁菌门)分布较均匀。4)土壤田间持水量、有机质和全磷、植被分布均匀度、Simpson多样性指数和丰富度指数是影响土壤细菌群落的主要因素。土壤因子(田间持水量、有机质和全磷)对灌木林和灌草丛非根际土细菌影响极显著,对根面和根际土细菌作用较小;植被因子(植被分布均匀度、Simpson多样性指数和丰富度指数)对乔木林(次生林和人工林)不同根际范围土壤细菌群落影响均达到极显著水平。5)新陈代谢功能在KEGG上的编码基因数量最多,是土壤细菌的�
【Objective】This study aimed to investigate the influence of different vegetation restoration patterns in mountainous areas of northern Hebei on the structural characteristics and functional diversity of rhizosphere soil bacterial community, and to explore the mechanisms of natural and artificial restoration measures on soil microorganism, so as to provide theoretical foundation for vegetation restoration and management in the region.【Method】The rhizoplane, rhizosphere and non-rhizosphere soils of the natural restored scrub-grassland, shrubbery and secondary forest, as well as the artificial restored forest were targeted, and high-throughput sequencing of V3-V4 fragment of bacterial 16 S rRNA was conducted by using the Illumina Miseq platform, to analyze the α,β and functional diversities of the soil bacteria, as well as the communities structure and their relationships with the environmental factors.【Result】 1) the α-diversity of soil bacteria among different restoration patterns was significantly different(P<0.05). The abundance and diversity of soil bacteria in secondary forest were all higher than those of artificial forest. To be specific, the scrub-grassland had higher diversity but the lowest abundance, while the shrubbery had higher abundance but the lowest diversity. There was no significant difference in the bacterial α-diversity among different rhizosphere ranges(P>0.05) The bacterial α-diversity in root surface and rhizosphere soils were relatively higher than that in non-rhizosphere soil. 2) There were certain similarities in soil bacterial community structure between secondary forests and artificial forests. However, there were big differences in soil bacterial community structure between secondary forests and scrub-grassland and shrubbery. Moreover, there was big difference in the soil bacterial community structure between root surface soil and rhizosphere and non-rhizosphere soils, while the bacterial community structure of rhizosphere soil was similar with that of non-rhizosphere
作者
王安宁
黄秋娴
李晓刚
徐学华
李玉灵
Wang Anning;Huang Qiuxian;Li Xiaogang;Xu Xuehua;Li Yuling(College of Forestry,Hebei Agricultural University Baoding 071000;College of Soil and Water Conservation,Beijing Forestry University Beijing 100083;Hebei Key Laboratory of Forest Germplasm Resources and Forest Protection Baoding 071000)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2019年第9期130-141,共12页
Scientia Silvae Sinicae
基金
国家重点研发计划项目(2016YFC0500806-07)
河北省高层次人才资助项目(132014010004)
关键词
冀北山区
植被恢复
ILLUMINA
Miseq测序
根际土
细菌群落结构
mountainous areas of northern Hebei
vegetation restoration
Illumina Miseq sequencing
rhizosphere soil
bacterial structure
