摘要
【目的】探究不同森林类型土壤细菌群落的多样性和组成,以及土壤细菌群落与土壤因子间的相关性,为掌握华南地区森林生态系统土壤细菌群落结构提供参考依据。【方法】采用高通量测序技术结合土壤理化性质试验,对土壤细菌群落结构进行分类鉴定,测定土壤pH、含水率、有机质(OM)、碱解氮(AN)、速效磷(AP)、速效钾(AK)、钙(Ca)、镁(Mg)、总酚、复合态酚及水溶性酚等理化指标;分析桉树林、马尾松林、马尾松石栎针阔混交林及桂花林土壤细菌群落多样性差异。【结果】通过测序从4种森林类型土壤样品中共得到内生细菌有效序列731289条,注释得到6320个操作分类单元(OTUs),共有2界36门85纲116目136科152属57种。其中,优势细菌门分别是变形菌门(Proteobacteria,33.16%)、放线菌门(Actinobacteria,30.24%)、酸杆菌门(Acidobacteria,16.83%)及绿弯菌门(Chloroflexi,6.71%),优势菌属分别是红游动菌属(Rhodoplanes,7.72%)、分枝杆菌属(Mycobacterium,2.82%)、Candidatus_Solibacter(1.57%)、慢生根瘤菌属(Bradyrhizobium,0.91%)及伯克霍尔德氏菌属(Burkholderia,0.67%)。不同森林生态系统中,桂花林的土壤细菌群落丰富度和多样性均最高,其次是桉树林和马尾松林,针阔混交林最低。冗余分析结果显示pH(r=0.943)、AK(r=0.861)对细菌群落结构均有极显著影响(P<0.01),其中,细菌在门分类水平上影响程度排序依次为p H>AK>复合态酚>总酚>Ca>Mg>AP>水溶性酚>OM>AN,在属分类水平上排序依次为pH>AK>Ca>Mg>AP>总酚>复合态酚>AN>水溶性酚>OM。【结论】4种森林类型土壤细菌群落多样性和丰度受土壤化学性质中pH和AK影响最大,部分门分类水平细菌受土壤酚类物质的影响较大。
【Objective】The objective of this study was to explore the diversity and composition of soil bacterial communities in different forest types,as well as the correlation between soil bacterial communities and soil factors,so as to provide reference for mastering the soil bacterial community structure in the forest ecosystem in southern China.【Method】High-throughput sequencing technology combined with soil physicochemical properties experiment was used to classify and identify the soil bacterial community structure,and the soil physicochemical indexes such as pH,soil moisture content,organic matter(OM),available nitrogen(AN),available phosphorus(AP),available potassium(AK),calcium(Ca),magnesium(Mg),total phenols,complex phenols and water-soluble phenols were determined.The differences in soil bacterial community diversity in Eucalyptus spp.forest,Pinus massoniana Lamb.forest,P.massoniana and Lithocarpus glaber(Thunb.)Nakai mixed coniferous and broad-leaved mixed forest and Osmanthus sp.forest were analyzed.【Result】A total of 731289 effective sequences of endophytic bacteria were obtained from soil samples of four forest types by sequencing,and 6320 operational taxonomic units(OTUs)were annotated,with a total of 57 species,152 genera,136 families,116 orders,85 classes,36 phyla in 2 kingdoms.Among them,the dominant bacterial phyla were Proteobacteria(33.16%),Actinobacteria(30.24%),Acidobacteria(16.83%)and Chloroflexi(6.71%),and the dominant bacterial gene-ra were Rhodoplanes(7.72%),Mycobacterium(2.82%),Candidatus_Solibacter(1.57%),Bradyrhizobium(0.91%)and Burkholderia(0.67%).Among the different forest ecosystems,the richness and diversity of soil bacterial communities were the highest in Osmanthus sp.forest,followed by Eucalyptus spp.forest,P.massoniana forest,and the richness and diversity of coniferous and broad-leaved mixed forest were the lowest.The results of redundancy analysis showed that pH(r=0.943)and AK(r=0.861)had extremely significant effects on bacterial community structure(P<0.01),among which
作者
杨希妤
田湘
吴庆标
YANG Xi-yu;TIAN Xiang;WU Qing-biao(College of Forestry,Guangxi University,Nanning,Guangxi 530004,China;Nanning Forest Park in Guangxi,Nanning,Guangxi 530031,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2024年第4期954-963,共10页
Journal of Southern Agriculture
基金
国家自然科学基金青年科学基金项目(32201431)
良凤江国家森林公园科技计划项目(LFJ2020001)。
关键词
高通量测序
森林类型
细菌群落
土壤理化性质
土壤酚酸类物质
high-throughput sequencing
forest type
bacterial community
soil physicochemical properties
soil phenolic acids
