摘要
目的探讨五叶泡醇提物抗大鼠急性痛风性关节炎的作用机制。方法采用注射尿酸钠的方法建立大鼠急性痛风性关节炎模型,测定踝关节肿胀度,进行步态观察及评分,检测各组大鼠踝关节滑膜组织肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、IL-6含量;qRT-PCR法检测大鼠踝关节滑膜组织核因子κB(NF-κB)P65、NF-κB抑制因子α(IκBα)mRNA的表达;观察大鼠踝关节滑膜组织病理学,运用免疫组化法检测大鼠滑膜NF-κB p65的表达。结果与模型组比较,五叶泡醇提物中、高剂量组踝关节肿胀度、步态评分较模型组有显著降低(P<0.05或P<0.01),滑膜组织TNF-α含量显著降低(P<0.05或P<0.01);五叶泡醇提物高剂量组滑膜组织IL-1β、IL-6含量显著降低(P<0.05),NF-κB P65 mRNA表达明显降低(P<0.05);五叶泡醇提物中、高剂量组大鼠滑膜组织IκBαmRNA表达明显升高(P<0.05)。病理结果显示五叶泡醇提物能改善滑膜组织病理损伤;免疫组化结果显示五叶泡醇提物高剂量组比模型组阳性着色细胞减少,染色变浅,平均光密度降低(P<0.01)。结论五叶泡醇提物可明显改善大鼠急性痛风性关节炎,其机制可能与抑制炎症因子表达及抑制NF-κB的活化有关。
This study was designed to investigate the effects of ethanol extract from Rubus rosaefolius Smith on acute gouty arthritis in rats.The model of acute gouty arthritis was established by monosodium urate in rats.The swelling degree of ankle and the scores of gait were measured;the levels of TNF-α,IL-1βand IL-6 were detected by ELISA;the mRNA levels of NF-κB P65 and its inhibitorα(IκBα)in synovial tissue of ankle were detected by qRT-PCR.Histopathological observation of synovial tissue was carried out,and NF-κB p65 in synovial tissue was detected by immunohistochemistry.Data showed that compared with the model group,middle and high doses of the ethanol extract could significantly decrease the swelling degree of ankle and the scores of gait(P<0.05 or P<0.01).Middle and high doses of the ethanol extract could significantly decrease the level of TNF-α(P<0.05 or P<0.01).High dose of the ethanol extract could significantly decrease the levels of IL-1βand IL-6(P<0.05 or P<0.01),and down-regulate the expression of NF-κB p65 mRNA(P<0.05).Middle and high doses of the ethanol extract could significantly up-regulate the expression of IκBαmRNA(P<0.05).The pathological results showed that the ethanol extract could alleviate the damage in synovial tissue;the results of immunohistochemistry showed that there were fewer positive staining cells,lighter staining and lower average optical density in the high dose group compared with the model group(P<0.01).In conclusion,the ethanol extract from Rubus rosaefolius Smith has mitigative effect on acute gouty arthritis in rats,which is probably related to its inhibitory effects on inflammatory factors and NF-κB.
作者
吕纪华
邓聿胤
王丽
刘瑛
苏华
LYU Jihua;DENG Yuyin;WANG Li;LIU Ying;SU Hua(Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards,Guangxi Institute of Chinese Medicine&Pharmaceutical Science,Nanning 530022,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2023年第4期312-319,共8页
Immunological Journal
基金
广西科技基地和人才专项(桂科AD17195002)
广西中药质量标准研究重点实验室自主研究课题青年骨干创新基金(桂中重自201412)。
关键词
五叶泡
急性痛风性关节炎
炎症因子
核因子ΚB
Rubus rosaefolius Smith
Acute gouty arthritis
Inflammatory factor
Nuclear factor kappa-B
