摘要
目的:观察虎杖提取物及其有效部位对痛风性关节炎的治疗作用。方法:SD大鼠随机等分为空白组,模型组,秋水仙碱组(0.28 mg·kg^(-1)),虎杖提取物高、中、低剂量组(260,130,65 mg·kg^(-1)),蒽醌有效部位组(11 mg·kg^(-1)),二苯乙烯有效部位组(650 mg·kg^(-1)),连续灌胃给药14 d,每天1次。第12天给药1 h后,采用大鼠右侧后肢踝关节腔注射尿酸钠溶液0.2 m L制备大鼠痛风性关节炎模型,造模后于不同时间点计算肿胀度。运用比色法测定大鼠血清中尿酸(UA)与黄嘌呤氧化酶(XOD)水平,苏木精-伊红(HE)染色法观察大鼠踝关节滑膜组织的病理变化。结果:与空白组比较,模型组大鼠踝关节肿胀度显著增加。与模型组比较,秋水仙碱组和虎杖提取物高、中剂量组均能显著减轻肿胀度,蒽醌有效部位组在2,4 h能显著降低肿胀度,二苯乙烯有效部位组在2,4,8,12,24,48 h均能显著降低肿胀度。与空白组比较,模型组UA和XOD水平显著增加。与模型组比较,虎杖提取物各剂量组能不同程度地降低UA和XOD水平,二苯乙烯和蒽醌有效部位可降低血UA和XOD水平。HE染色发现虎杖提取物及其有效部位能改善滑膜组织病理的变化。结论:虎杖提取物的抗痛风性关节炎可能是二苯乙烯和蒽醌共同作用的结果。
Objective： To observe the effect of Polygoni Cuspidati Rhizoma et Radix extract and its effective parts on gouty arthritis. Method： SD rats were randomly divided into 8 groups,such as the blank group,model group,colchicine group（ 0. 28 mg·kg-1）,high,middle and low（ 260,130,65 mg·kg-1） dose group of Polygoni Cuspidati Rhizoma et Radix extract, anthraquinone effective part group（ 11 mg·kg-1）,stilbene effective part group（ 650 mg·kg-1）,continuous intragastric administration of 14 days for once a day,after administration of 1 h in the twelfth day,rats were injected with 0. 2 m L of uric acid sodium solution at the right hind limb,the right hind limb’s circumference of rats were measured at 2,4,8,12,24,48 h in the same site,the degree of swelling was calculated. Colorimetric method was adopted to determine the level of uric acid（ UA）and xanthine oxidase（ XOD） in rat serum. Hematoxylin-eosin（ HE） staining was employed to observe the pathological changes of the rat ankle joint synovial tissue. Result： Compared with the blank group,rats ankle swelling degree of the model group increased significantly. Compared with the model group,high and middle dose group of Polygoni Cuspidati Rhizoma et Radix extract and colchicine group can significantly reduce swelling degree,anthraquinone effective part group at 2,4 h can significantly reduce swelling degree,stilbene effective part group in 2,4,8,12,24,48 h can significantly reduce swelling degree. Compared with the blank group,UA and XOD levels in serum were significantly increased in the model group. Compared with the model group,Polygoni Cuspidati Rhizoma et Radix extract groups and its effective part groups can significantly reduce UA and XOD levels. Polygoni Cuspidati Rhizoma et Radix extract and its effective part can improve the pathology of synovial tissue. Conclusion： Anti-gouty arthritis of Polygoni Cuspidati Rhizoma et Radix extract may be the result of joint action of stilbene and anthraquinone.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第19期111-115,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省中医药管理局项目(QZYY-2014-030)
贵州省科技厅项目(黔科合LH字[2015]7548号)
遵义医学院科研项目(遵医院办发[2014]10号)
关键词
虎杖
痛风性关节炎
尿酸
黄嘌呤氧化酶
二苯乙烯类
蒽醌类
Polygoni Cuspidati Rhizoma et Radix
gouty arthritis
uric acid
xanthine oxidase
stilbene
anthraquinone
