摘要
为延长甜瓜未授粉子房取材时间,克服甜瓜子房取材受生长季节、发育期限制的缺点,以厚皮甜瓜晚熟品种众云18和早熟品种锦绣的子房为试材,对其进行超低温保存和恢复培养研究。结果表明,4℃暗处理的甜瓜子房随着预培养和保护液(PVS2)处理时间的增加,子房的存活率呈先增高后降低的趋势,其中,预培养24 h和PVS2处理30 min时效果最佳,子房存活率均在82%以上;子房经过-196℃超低温保存后,在38、40、42℃卸载液解冻5 min时,子房存活率没有明显差异;超低温保存60 d后的子房,经过恢复培养后,众云18和锦绣子房最高存活率分别达到80.83%、82.43%。采用包埋法和玻璃化法结合的改良超低温保存法可以有效解决甜瓜大孢子培养供体无法持续获得的问题,加速了甜瓜单倍体培育的进程。
In order to prolong the time of melon unpollinated ovary sampling and overcome the disadvantage that the ovary of melon sampling is limited by the growing season and development period.In this experiment,the ovaries of the late-maturing variety Zhongyun-18 and the early-maturing variety Jinxiu were used as test materials for cryopreservation and recovery culture.The results showed that:With the increase of preculture and protection solution(PVS2)treatment time,the survival rate of melon ovary treated at 4℃in the dark showed increase first and then decrease,and the best results were obtained after 24 h of preculture and 30 min of PVS2 treatment,and the survival rate of ovary was above 82%.After cryoppreservation at-196℃,there was no significant difference in the survival rate of ovary when the unloading solution was thawed at 38,40℃and 42℃for 5 min.After 60 days of cryopreservation,the highest survival rates of Zhongyun-18 and Jinxiu ovaries were 80.83%and 82.43%,respectively.In this study,the improved cryopreservation method combining the embedding method and vitrification method could effectively solve the problem that melon megaspore culture materials cannot be continuously obtained,and accelerate the process of melon haploid cultivation.
作者
李海伦
高宁宁
李晓慧
康利允
常高正
梁慎
王慧颖
徐小利
赵卫星
LI Hailun;GAO Ningning;LI Xiaohui;KANG Liyun;CHANG Gaozheng;LIANG Shen;WANG Huiying;XU Xiaoli;ZHAO Weixing(Horticulture Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)
出处
《中国瓜菜》
CAS
北大核心
2023年第4期76-80,共5页
China Cucurbits And Vegetables
基金
财政部和农业农村部:国家现代农业产业技术体系(CARS-25)
河南省西甜瓜产业技术体系建设项目(HARS-22-10-G1)
河南省科技攻关项目(222102110208)
河南省农业科学院自主创新项目(2022ZC25)。
关键词
甜瓜
未授粉子房
超低温保存
Melon
Unpollinated ovary
Cryopreservation
