摘要
目的探讨慢性肾小球肾炎(CGN)患者血清二胺氧化酶(DAO)、D-乳酸(D-LA)表达与辅助型T细胞1(TH1)/辅助型T细胞2(TH2)失衡的关系。方法选取2021年1月至2022年1月于中国人民解放军总医院确诊为CGN的患者108例作为CGN组,按照慢性肾脏疾病(CKD)分期将患者按照疾病的严重程度分组,32例为CKD1~2组,40例为CKD3~4组,36例为CKD5组。选取本院同期85名正常的体检健康人群为对照组。检测并比较两组以及CGN组不同疾病严重程度患者TH1/TH2、DAO、D-LA水平以及TH1细胞分泌的白介素-2(IL-2)和干扰素-γ(IFN-γ)及TH2细胞分泌的白介素-4(IL-4)和白介素-10(IL-10)的含量。采用Pearson相关性分析DAO、D-LA水平及二者与TH1、TH2、TH1/TH2、IL-2、IFN-γ、IL-4、IL-10的相关性。结果CGN组患者血清DAO和D-LA水平较对照组显著升高,差异具有统计学意义(t=6.917、19.051,P<0.05);DAO和D-LA的水平:CKD5组>CKD3~4组>CKD 1~2组,差异有统计学意义(F=16.364、F=21.333,P<0.05);Pearson相关性分析结果显示CGN患者DAO和D-LA水平呈正相关关系(r=0.488,P<0.001)。CGN组TH1、TH1/TH2的比例以及IL-2、IFN-γ的水平显著高于对照组,差异有统计学意义(t=5.703、10.539、10.435、7.737,P<0.05),TH2比例以及IL-4、IL-10水平显著低于对照组,差异具有统计学意义(t=6.831、11.202、25.630,P<0.05)。随着CGN组疾病严重程度升高,TH2比例以及IL-4、IL-10水平明显降低,TH1、TH1/TH2比例以及IL-2、IFN-γ水平明显升高(P<0.05);CGN患者血清DAO和D-LA水平与TH1、TH1/TH2、IL-2、IFN-γ呈正相关,与TH2、IL-4、IL-10水平呈负相关(P<0.05)。结论CGN患者血清DAO、D-LA呈高水平,推断二者水平变化与TH1/TH2失衡有关。
Objective To investigate the relationship between expression of serum diamine oxidase(DAO),D⁃lactate(D⁃LA)and the imbalance of T helper 1(TH1)/helper 2(TH2)in patients with chronic glomerulonephritis(CGN).Methods A total of 108 patients diagnosed with CGN in the General Hospital of Chinese People's Liberation Army from January 2021 to January 2022 were selected as the CGN group.According to the stage of chronic kidney disease(CKD),the patients were divided into the CKD1⁃2 group(n=32),the CKD3⁃4 group(n=40)and the CKD⁃5 group(n=36).A total of 85 healthy people with normal physical examination were selected as the control group.The levels of TH1/TH2,DAO and D⁃LA as well as the contents of interleukin⁃2(IL⁃2)and interferon⁃γ(IFN⁃γ)secreted by TH1 cells and interleukin⁃4(IL⁃4)and interleukin⁃10(IL⁃10)secreted by TH2 cells were detected and compared in the two groups and the CGN group with different disease severity.Pearson correlation was used to analyze the levels of DAO and D⁃LA and their correlation with TH1,TH2,TH1/TH2,IL⁃2,IFN⁃γ,IL⁃4 and IL⁃10.Results The levels of serum DAO and D⁃LA in the CGN group were significantly higher than those in the control group,the difference was statistically significant(t=6.917,P<0.05;t=19.051,P<0.05).The levels of DAO and D⁃LA were as follows:CKD5 group>CKD3⁃4 group>CKD1⁃2 group,the difference was statistically significant(F=16.364,P<0.05;F=21.333,P<0.05);Pearson correlation analysis showed a positive correlation between DAO and D⁃LA levels in CGN patients(r=0.488,P<0.001).The proportions of TH1,TH1/TH2 and levels of IL⁃2 and IFN⁃γin the CGN group were significantly higher than those in the control group(t=5.703,10.539,10.435,7.737,P<0.05),and the proportions of TH2 and levels of IL⁃4 and IL⁃10 in the CGN group were significantly lower than those in the control group,the difference was statistically significant(t=6.831,11.202,25.630,P<0.05).With the increase of disease severity in the CGN group,the proportion of TH2 and
作者
韩博
于敏
熊锡山
HAN Bo;YU Min;XIONG Xishan(Department of Nephrology,the Fifth Medical Centre,Chinese PLA General Hospital,Beijing,China,100071;Department of Nephrology,Suzhou Hospital Affiliated to Anhui Medical University,Suzhou,Anhui,China,234000)
出处
《分子诊断与治疗杂志》
2023年第3期387-391,共5页
Journal of Molecular Diagnostics and Therapy
基金
安徽省高校自然科学研究项目(KJ2021A0346)。
