摘要
目的从表观遗传学角度探讨ALKBH5基因甲基化与乙肝疫苗(HepB)应答水平的关联性。方法选择2016年1-12月间在广西壮族自治区妇幼保健院、南宁市妇幼保健院、南宁市第一人民医院已严格按照HepB免疫接种程序完成初次免疫的广西汉族263名8~9月龄儿童作为研究对象,检测乙肝五项等指标,采用非匹配病例对照研究方法,将抗-HBs<100 mIU/mL者纳入病例组,抗-HBs≥100 mIU/mL者纳入对照组。采用Panel多重PCR技术、重亚硫酸盐转化测序技术和高通量测序等技术,检测ALKBH5基因CpG位点DNA甲基化情况。结果病例组ALKBH5_1基因92位点、ALKBH5_3基因97位点的DNA甲基化水平均高于对照组,差异均有统计学意义(P=0.024、0.013)。经非条件logistic回归分析显示ALKBH5_1基因92位点[OR(L95~U95):2.0(1.08~3.72)]与ALKBH5_3基因97位点[OR(L95~U95):0.6(0.38~0.93)]DNA甲基化与HepB应答水平差异有统计学意义(P<0.05)。结论ALKBH5_1基因92位点DNA甲基化可能增加广西汉族儿童发生HepB免疫低/无应答的风险,而ALKBH5_3基因的97位点的甲基化可能降低发生HepB免疫低/无应答的风险。
Objective Explored the relationship between ALKBH5 gene methylation and the response level of hepatitis Bvaccine(Hep B)from the perspective of epigenetics.MethodsThe 263 Han children from 8 to 9 months old in Guangxiwho had completed the initial immunization in strict accordance with the Hep B vaccination procedure were selected as theresearch objects.Five indicators of hepatitis B and other indicators were detected.The non-matched case-control studymethod was used to include those with anti-HBs<100 m IU/m L in the case group;those with anti-HBs≥100 m IU/m L wereincluded in the control group.Panel multiple PCR technology,bisulfite conversion sequencing technology and high-throughput sequencing technology were used to detect DNA methylation at the Cp G site of ALKBH5 gene.ResultsThemethylation level of the 92 site of ALKBH5_1 gene and the DNA methylation level of the 97 site of ALKBH5_3 gene were allhigher than that of the control group,and the differences were statistically significant(P=0.024,0.013).Unconditionallogistic regression analysis showed that DNA methylation of the 92 site of ALKBH5_1 gene[OR(L95-U95):2.0(1.08-3.72)]and the 97 site of ALKBH5_3 gene[OR(L95-U95):0.6(0.38-0.93)]and the response level of HepB showedsignificant differences(P<0.05).ConclusionDNA methylation at position 92 of ALKBH5_1 gene might increase the riskof Hep B immuno hypo/non-responsiveness in Han children in Guangxi,while methylation at position 97 of ALKBH5_3 genemight reduce the risk of HepB immune hypo/non-responsiveness.
作者
李嘉铃
陆玉英
董柏青
陈钦艳
胡莉萍
王超
苏永健
李海
LI Jia-ling;LU Yu-ying;DONG Bai-qing;CHEN Qin-yan;HU Li-ping;WANG Chao;SU Yong-jian;LI Hai(Department of Epidemiology,School of Public Health and Management,Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530200,China;Guangxi Key Laboratory of Integrated Traditional Chinese and Western Medicine Translational Medicine for High-incidence Infectious Diseases,Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530200,China;Guangxi Center for Disease Control and Prevention,Guangxi Zhuang Autonomous Region Guangxi Key Laboratory of Viral Hepatitis Prevention and Treatment,Nanning,Guangxi 530029,China;Department of Biochemistry,School of Basic Medicine,Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530200,China)
出处
《热带医学杂志》
CAS
2022年第11期1498-1501,共4页
Journal of Tropical Medicine
基金
国家自然科学基金(81760604,81360443,81703283)
广西自然科学基金(2013GXNSFBA019194,2017GXNSFBA198086)
广西中医药大学研究生教育创新计划资助项目(YCSZ2020020,YCXJ2021030)
