摘要
目的探讨Th1和Th2细胞因子基因多态性与新生儿乙型肝炎(乙肝)疫苗(HepB)低免疫应答的关系。方法在广西两家医院招募已完成HepB全程免疫的8-9月龄汉族儿童,采用微粒子酶联免疫测定法测定血清乙肝表面抗体(HBsAb),抗体浓度≥10mIU/L且<100mIU/L为低应答,≥1000mIU/L为高应答。采用SNPscanTM多重单核苷酸多态性(SNP)分型技术检测细胞因子,分析基因位点、基因型、等位基因与HepB免疫应答水平的关联性。结果本研究纳入HepB低应答儿童88名、高应答儿童132名;共筛选出Th1和Th2细胞因子12个基因52个位点。Logistic回归分析显示,IL18基因rs3882891位点T/T基因型、IL12A基因rs583911位点A等位基因、IFN-γ基因rs2069728位点C等位基因的低应答比例高[OR(95%CI):2.72(1.33-5.57);1.81(1.15-2.85);2.01(1.07-3.79)],IL1B基因rs16944位点A等位基因的低应答比例低[OR(95%CI):0.64(0.43-0.95)]。结论IL18基因rs3882891位点T/T基因型、IL12A基因rs583911位点A等位基因、IFN-γ基因rs2069728位点C等位基因可能与汉族儿童HepB初次免疫低应答有关,而IL1B基因rs16944位点A等位基因可能是其保护因素。
Objective To explore the association of Th1 and Th2 cytokine gene polymorphisms with low immune response to hepatitis B vaccine(HepB) in newborns. Methods We recruited 8-9-month-old Han children who had received full-series HepB vaccination in two hospitals of Guangxi. We tested serum samples for hepatitis B surface antibody(HBs Ab) by microparticle enzyme-linked immunoassay. We defined low response as an antibody concentration ≥10 mI U/L but <100 mI U/L, and high response as≥1 000 miu/L. We detected cytokines by the SNPscan TM multiple single nucleotide polymorphism(SNP) typing technique to determine associations of gene locus, genotypes, and alleles with HepB immune response. Results We included 88 low-response and 132 high-response children in the study. A total of 52 sites in 12 Th1 and Th2 cytokine genes were identified. Logistic regression showed that proportions of low-response children with T/T genotype at rs3882891 of IL18 gene, A allele at rs583911 of IL12 A gene, and C allele at rs2069728 of IFN-γ gene were high(OR[95%CI]: 2.72[1.33-5.57], 1.81[1.15-2.85], and 2.01[1.07-3.79]), and the proportion of low-response children with A allele at rs16944 of IL1 B gene was low(OR[95%CI]: 0.64[0.43-0.95]). Conclusion T/T genotype at rs3882891 of IL18 gene, A allele at rs583911 of IL12 A gene, and C allele at rs2069728 of IFN-γ gene may be associated with low immune response to primary HepB vaccination in Han children, while A allele at rs16944 of IL1 B gene may be a protective factor.
作者
李嘉铃
姜露
杨庆利
陈钦艳
居昱
吴琪俊
董柏青
李海
Li Jialing;Jiang Lu;Yang Qingli;Chen Qinyan;Ju Yu;Wu Qijun;Dong Baiqing;Li Hai(School of Public Health and Management,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;Guangxi Key Laboratory of Translational Medicine for Treating High-incidence Infectious Diseases with Integrative Medicine,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;Guangxi Key Laboratory for Control and Prevention of Viral Hepatitis,Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention,Nanning 530028,Guangxi,China;Department of Emergent Infectious Disease Control and Prevention,Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention,Nanning 530028,Guangxi,China)
出处
《中国疫苗和免疫》
北大核心
2020年第3期253-260,共8页
Chinese Journal of Vaccines and Immunization
基金
国家自然科学基金项目(81760604,81360443,81560546)
广西自然科学基金项目(2013GXNSFBA019194)
广西中医药大学研究生教育创新计划资助项目(YCSZ2020020)。
