摘要
目的 分析肺炎支原体肺炎(MPP)患儿炎症细胞因子和免疫细胞与小气道功能的相关性,了解炎症状态和免疫细胞对小气道功能损伤的影响。方法 选择2015年6月—2020年6月绍兴第二医院医共体总院儿科收治的诊断为MPP的患儿107例,其中31例出现喘息,同时选择同期体检健康的50例正常儿童作为对照组。检测所有患儿的肺功能,包括用力肺活量(FVC)、第1秒用力呼气量(FEV_(1))以及小气道功能指标[包括呼吸初期瞬间流量(FEF75%)、呼吸中期瞬间流量(FEF50%)、呼吸后期瞬间流量(FEF25%)及最大呼气中段流量(MMEF)]。采用酶联免疫吸附法检测所有儿童的炎症细胞因子水平,包括白细胞介素-4(IL-4)、白细胞介素-6(IL-6)及γ-干扰素(IFN-γ)。检测免疫细胞,包括CD3^(+)T细胞、CD4^(+)T细胞、CD8^(+)T细胞以及CD4^(+)/CD8^(+)T细胞比值。结果 喘息组儿童IL-4、IL-6及IFN-γ水平分别为(41.73±5.16)pg/ml、(26.93±3.32)pg/ml及(683.15±80.74)ng/L;未喘息组儿童IL-4、IL-6及IFN-γ水平分别为(26.91±4.47)pg/ml、(17.84±2.74)pg/ml及(406.18±78.16)ng/L;对照组儿童IL-4、IL-6及IFN-γ水平分别为(15.84±3.14)pg/ml、(7.43±1.08)pg/ml及(236.75±57.13)ng/L。3组儿童IL-4、IL-6及IFN-γ水平比较差异均有统计学意义(F=12.746,18.578,10.875,均P<0.05)。喘息组儿童IL-4、IL-6及IFN-γ水平均显著高于未喘息组和对照组,未喘息组儿童IL-4、IL-6及IFN-γ水平均显著高于对照组,差异均有统计学意义(均P<0.05)。3组儿童CD3^(+)T细胞水平比较差异有统计学意义(P<0.05),喘息组儿童CD3^(+)T细胞水平高于未喘息组和对照组,未喘息组儿童CD3^(+)T细胞水平高于对照组,差异均有统计学意义(P<0.05)。3组儿童FVC、FEV_(1)、FEF25%、FEF50%、FEF75%及MMEF水平比较差异均有统计学意义(均P<0.05)。喘息组儿童FVC、FEV_(1)、FEF25%、FEF50%、FEF75%及MMEF水平均显著低于未喘息组和对照组,未喘息组儿童FVC、FEV_(1)、FEF25%、FEF5
Objective To analyze the correlation between cytokines, immune cells and small airway function in children with Mycoplasma pneumoniae pneumonia(MPP), investigate the effects of inflammation state and immune cells on small airway injury. Methods From June 2015 to June 2020,107 children diagnosed as MPP in General Hospital of Medical Treatment Combination of the Second Hospital of Shaoxing were selected. Among them, 31 children had wheezing. Meanwhile, 50 healthy children in other hospital during the same period were selected as control group. Pulmonary function of all the children was tested, including forced vital capacity(FVC), forced expiratory volume in the first second(FEV_(1)), and small airway function indexes [including forced expiratory flow at 75% of FVC exhaled(FEF75%), forced expiratory flow at 50% of FVC exhaled(FEF50%), forced expiratory flow at 25% of FVC exhaled(FEF25%), and maximal mid-expiratory flow(MMEF)]. The levels of inflammatory cytokines in all the children were detected, including interleukin-4(IL-4), interleukin-6(IL-6), and gamma-interferon(IFN-γ). The levels of immune cells in all the children were detected, including CD3^(+)T cells, CD4^(+)T cells, CD8^(+)T cells, and CD4^(+)/CD8^(+)T cell ratio. Results The levels of IL-4,IL-6 and IFN-γ in wheezing group were(41.73±5.16)pg/ml,(26.93±3.32)pg/ml, and(683.15±80.74)ng/L, respectively;the levels of IL-4,IL-6 and IFN-γ in non-wheezing group were(26.91±4.47)pg/ml,(17.84±2.74)pg/ml, and(406.18±78.16)ng/L, respectively;the levels of IL-4,IL-6 and IFN-γ in control group were(15.84±3.14)pg/ml,(7.43±1.08)pg/ml, and(236.75±57.13)ng/L, respectively. There were statistically significant differences in the levels of IL-4,IL-6 and IFN-γ among the three groups(F=12.746,18.578,10.875,P<0.05). The levels of IL-4,IL-6 and IFN-γ in wheezing group were statistically significantly higher than those in non-wheezing group and control group, the levels of IL-4,IL-6 and IFN-γ in non-wheezing group were statistically significantly higher than tho
作者
张炫炜
陈俊松
汤昱
ZHANG Xuan-wei;CHEN Jun-song;TANG Yu(General Hospital of Medical Treatment Combination of the Second Hospital of Shaoxing,Shaoxing,Zhejiang 312000,China)
出处
《中国妇幼保健》
CAS
2023年第6期1024-1028,共5页
Maternal and Child Health Care of China
基金
河南省医学科技攻关计划项目(201403258)。
关键词
肺炎支原体肺炎
小气道功能
炎症细胞因子
免疫细胞
Mycoplasma pneumoniae pneumonia
Small airway function
Inflammatory cytokine
Immune cell
