摘要
目的探讨黄芪多糖对雄性小鼠急性放射性肠损伤的辐射防护机制。方法将50只C57BL/6J雄性小鼠随机分为空白对照组、模型组、照射+黄芪多糖低剂量组、照射+黄芪多糖中剂量组、照射+黄芪多糖高剂量组。模型组和照射+黄芪多糖低、中、高剂量组采用直线加速器做腹部照射(X线,12Gy)建模。于照射后3日取小鼠小肠组织,用HE染色法观察小肠黏膜病理情况,并测量小肠绒毛高度和隐窝深度;用ELISA法测定小肠组织中SOD、MDA、MPO的水平;用IHC法检测小肠组织中p53的表达水平;用RT-PCR法检测小肠组织中PI3K、p-Akt、p53 mRNA的表达水平。结果与空白对照组比较,模型组小鼠疾病活动指数评分升高,小肠黏膜病理损伤加重,绒毛高度降低,隐窝深度缩短,小肠组织SOD活性下降,MDA和MPO水平升高,p53、PI3K、p-Akt及p53 mRNA表达水平升高,差异均有统计学意义(P<0.05);与模型组比较,随着黄芪多糖剂量的增加,经过各剂量黄芪多糖作用的照射组小鼠疾病活动指数评分逐渐降低,小肠黏膜病理损伤减轻,绒毛高度和隐窝深度较模型组呈恢复态势,小肠组织SOD活性逐渐升高,MDA和MPO水平逐渐下降,p53、PI3K、p-Akt及p53 mRNA表达水平逐渐下降,差异均有统计学意义(P<0.05)。结论在雄性小鼠急性放射性肠损伤中,黄芪多糖通过调节PI3K/Akt信号通路减轻炎性损伤发挥辐射防护作用。
Objective To explore the radiation protective mechanism of Astragalus polysaccharides against acute radiation caused intestinal injury in male mice.Methods Fifty C57BL/6J male mice were randomly divided into blank control group,model group,irradiation+low dose,middle dose and high dose of Astragalus polysaccharide group.The model group and the irradiation+astragalus polysaccharide low,middle and high dose groups were modeled by abdominal irradiation(X-ray,12Gy)with linear accelerator.On the 3rd day after irradiation,the small intes tine tissues of mice were taken,and the pathological conditions of small intestine mucosa were observed by HE staining,and the villi height and crypt depth of small intestine were measured.The levels of Superoxide Dismutase(SOD),Malondialdehyde(MDA)and Myeloperoxidase(MPO)in small intestine were detected by enzyme linked immune-absorbent assay(ELISA).The expression level of p53 in small intestine was detected by IHC.The expression levels of PI3K,p-Akt and p53 mRNA in small intestine were detected by RT-PCR.Results Compared with the blank control group,the disease activity index score of mice in the model group increased,the pathological damage of small intestinal mucosa worsened,the villi height decreased and the crypt depth shortened,the SOD activity in small intestinal tissue decreased,the MDA and MPO levels increased,and the expression levels of p53,PI3K,p-Akt and p53 mRNA increased,with statistical significance(P<0.05).Compared with the model group,with the increase of the dosage of Astragalus polysaccharides,the disease activity index score of mice irradiated by various doses of Astragalus polysaccharides gradually decreased,the pathological damage of small intestinal mucosa was alleviated,the villi height and crypt depth were restored,the activity of SOD in small intestinal tissue gradually increased,the levels of MDA and MPO gradually decreased,and the expression levels of p53,PI3K,p-Akt and p53 mRNA gradually decreased,with statistical significance(P<0.05).Conclusion Astragalus
作者
高悦
冯瑞兴
赵兴
杨依
郝彦惠
陈凡
Gao Yue;Feng Ruixing;Zhao Xing;Yang Yi;Hao Yanhui;Chen Fan(Department of medicine,Qinghai University,Xining 810001,China;Affiliated Hospital of Qinghai University,Qinghai University Cancer Hospital,Xining 810001,China)
出处
《中国高原医学与生物学杂志》
CAS
2023年第2期113-120,共8页
Journal of Chinese High Altitude Medicine & Biology
基金
青海省卫生健康委员会项目(2020-wjzdx-50)。
